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Agrisera
ClpB-P/ClpB3 Polyclonal Antibody
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FIGURE: 1 / 1
ClpB-P/ClpB3 Antibody (AS09-459) in WB
10 µg of total protein from 7 day old Arabidopsis plants grown at control conditions, 22°C wild type, Columbia-9 (1), hot 1-3, HSP 101 null (2), clp Bp-1, Clp Bp null (3) and heat shocked at 38°C for 1.5 hour wild type, Columbia-9 (4), hot 1-3, HSP 101 null (5), clp Bp-1, Clp Bp null (6) were separated on 7-16% SDS-PAGE and blotted overnight to nitrocellulose membrane. Blots were blocked immediately following transfer in for 1 h at room temperature with agitation. Blots were incubated in Agrisera anti-Clp B-P antibody (AS09 459) at a dilut... View More
Product Details
AS09-459
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from ClpB-P of Arabidopsis thaliana.
Conjugate
Form
Lyophilized
Storage buffer
whole serum
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 100 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
ClpB3 encodes a nucleus-encoded, chloroplast-targeted AAA+ chaperone/disaggregase that is imported into the chloroplast stroma as a processed preprotein with an N-terminal transit peptide; under ambient conditions it is broadly stromal, but during heat stress it can relocalize into discrete stromal foci near the thylakoid membrane system, consistent with a role at sites of aggregate deposition. Structurally, ClpB3 is a class I Hsp100/Clp chaperone that assembles into an ATP-dependent homohexameric ring and contains the characteristic N-terminal domain, an inserted coiled-coil middle domain, and two tandem AAA+ ATPase modules with Walker-type nucleotide-binding motifs that power substrate engagement and threading through a central pore. Functionally, ClpB3 supports chloroplast proteostasis by recognizing and resolubilizing stress-denatured and aggregated proteins using ATP hydrolysis, thereby promoting recovery of chloroplast protein homeostasis after heat stress; genetic and biochemical data indicate it is required in vivo for efficient clearance of heat-induced chloroplast protein aggregates and contributes to cellular thermotolerance, and purified plant ClpB3 can directly reactivate aggregated model substrates in an ATP-dependent manner.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: APG6; AT5G15450; AtCLPB3; CLPB-P; T20K14.60; T20K14_60
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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