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Cofilin Antibody (PA5-95176) in ICC/IF
Immunocytochemistry analysis of Cofilin using anti-Cofilin antibody (Product # PA5-95176) . Cofilin was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Cofilin antibody (Product # PA5-95176) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence micr... View More
Product Details
PA5-95176
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E.coli-derived human Cofilin recombinant protein (Position: A2-L166).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 5mg BSA
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2806981
Product Specific Information
Human Cofilin shares 99% amino acid (aa) sequences identity with both mouse and rat Cofilin.
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Hela whole cell, monkey COS-7 whole cell, human HEK293 whole cell, human U-87MG whole cell, human HepG2 whole cell, human PC-3 whole cell, human THP-1 whole cell, human A549 whole cell, rat heart tissue, rat spleen tissue, rat lung tissue, rat C6 whole cell, mouse spleen tissue, mouse NIH/3T3 whole cell, mouse HEPA1-6 whole cell. IHC: mouse lung tissue, rat lung tissue, human intestinal cancer tissue, human mammary cancer tissue IHC-F: human placenta tissue. ICC/IF: U20S cell. Flow: U20S cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
Target Information
Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 18 kDa phosphoprotein; CFL-1; COF1; cofilin 1 (non-muscle); Cofilin, non-muscle isoform; Cofilin-1; Cofilin1; epididymis secretory protein Li 15; p18
Gene Aliases: AA959946; CFL; CFL1; Cof; cofilin; HEL-S-15; n-cofilin
UniProt ID: (Human) P23528, (Rat) P45592, (Mouse) P18760
Entrez Gene ID: (Human) 1072, (Rat) 29271, (Mouse) 12631
cytoskeleton organization
Rho protein signal transduction
response to virus
regulation of cell morphogenesis
actin cytoskeleton organization
actin filament depolymerization
negative regulation of apoptotic process
positive regulation by host of viral process
regulation of dendritic spine morphogenesis
mitotic cytokinesis
neural crest cell migration
neural fold formation
protein phosphorylation
protein import into nucleus
actin filament organization
establishment of cell polarity
cell projection organization
positive regulation of actin filament depolymerization
response to amino acid
negative regulation of cell size
movement of cell or subcellular component
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