Invitrogen

Cofilin Polyclonal Antibody

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Datasheet

Protocols

Questions & Answers

Tech Support

Antibody Testing Data (9)

FIGURE: 1 / 9

Cofilin Antibody (PA5-95176) in WB

Western blot analysis of Cofilin in, Lane 1: human Hela whole cell lysates, Lane 2: monkey COS-7 whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human U-87MG whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: human PC-3 whole cell lysates, Lane 7: human THP-1 whole cell lysates, Lane 8: human A549 whole cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing... View More

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Cofilin Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of Cofilin using anti-Cofilin antibody (Product # PA5-95176) . Cofilin was detected in a section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-Cofilin antibody (Product # PA5-95176) overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.

Cofilin Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Cofilin in paraffin-embedded section of human intestinal cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Cofilin antibody (Product # PA5-95176) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Cofilin Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Immunohistochemical analysis of Cofilin in frozen section of human placenta tissues. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/mL rabbit anti-Cofilin antibody (Product # PA5-95176) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.

Cofilin Antibody in Flow Cytometry (Flow) — Flow Cytometry of Cofilin in U2OS cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with Cofilin Polyclonal Antibody (Product # PA5-95176) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

Product Details

PA5-95176

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

0.5-1 µg/mL

Immunocytochemistry (ICC/IF)

2 µg/mL

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

ELISA (ELISA)

0.1-0.5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Non-human primate, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E.coli-derived human Cofilin recombinant protein (Position: A2-L166).

View immunogen

Conjugate

Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Affinity chromatography

Storage buffer

PBS with 5mg BSA

Contains

0.05mg sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2806981

Product Specific Information

Human Cofilin shares 99% amino acid (aa) sequences identity with both mouse and rat Cofilin.

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Target Information

Cofilin binds to F-actin and exhibits pH-sensitive F-actin depolymerizing activity. Regulates actin cytoskeleton dynamics. Important for normal progress through mitosis and normal cytokinesis. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required for the up-regulation of atypical chemokine receptor ACKR2 from endosomal compartment to cell membrane, increasing its efficiency in chemokine uptake and degradation. Required for neural tube morphogenesis and neural crest cell migration.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: 18 kDa phosphoprotein; CFL-1; COF1; cofilin 1 (non-muscle); Cofilin, non-muscle isoform; Cofilin-1; Cofilin1; epididymis secretory protein Li 15; p18

Gene Aliases: AA959946; CFL; CFL1; Cof; cofilin; HEL-S-15; n-cofilin

UniProt ID: (Human) P23528, (Rat) P45592, (Mouse) P18760

Entrez Gene ID: (Human) 1072, (Rat) 29271, (Mouse) 12631

Molecular Function: non-motor actin binding protein