Western blot detection of Collagen I in 100µg per lane of (lane 1) Wistar rat hepatic stellate cells (HSC) in control (GFP-transduced) and (lane 2) PPARg-transduced cell lysates. Protein staining using a Collagen I polyclonal antibody (Product # PA1-28530) at a dilution of 0.2–2µg/10 ml followed by incubation overnight at 4°C. Secondary detection was performed using an HRP-conjugated rabbit secondary antibody at 1 µg/10 ml overnight at 4°C. Samples were blocked TBS with 5% Non-fat milk. Predicted/Observed size: 138.9 kDa for Collagen I.
|Tested species reactivity||Bovine, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Collagen Type I from adult human and bovine tissues.|
|Purification||Antigen affinity chromatography|
|Storage buffer||BBS, pH 8.0, with 10mg/ml BSA, 5mM EDTA|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:5000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28530 detects Collagen I from bovine, human samples.
PA1-28530 has been successfully used in immunohistochemistry (paraffin tissue), immunoprecipitation, Western blot, and ELISA applications.
The PA1-28530 immunogen is collagen type I from adult human and bovine tissues.
For extended storage add 50% glycerol, aliquot and store at -20°C or below, avoiding freeze-thaw cycles.
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.