|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Collagen type I from human placenta.|
|Storage buffer||PBS with 1% dextran, 1% mannitol|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:10-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This product requires antigen retrieval using 1mg/ml pepsin in 0.5M acetic acid for 2 hours at 37°C and multiple buffer washes prior to staining on paraffin sections.
Reconstitute with 0.5 ml of distilled water. Prior to reconstitution, store at 4°C. After reconstitution, store undiluted at -20°C, avoiding freeze/thaw cycles. For long term storage the addition of 1% BSA and 0.09% sodium azide is recommended.
Collagen is located in the extracellular matrix of connective tissues. It is part of the interacting network of proteoglycans and proteins that provides a structural framework for both soft and calcified connective tissues. Type I collagen (95 kDa) is found in bone, cornea, skin and tendon. Mutations in the encoding gene are associated with osteogenesis imperfecta, Ehlers Danlos syndrome, and idiopathic osteoporosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.