Immunohistochemical analysis of Collagen II in formalin-fixed, paraffin-embedded human bronchiolar cartilage tissue using a Collagen II polyclonal antibody (Product # PA1-26206) at a dilution of 1:400 for 45 min at room temperature. Though not shown, faint to moderate staining of tonsillar squamous epithelium, prostatic stroma, breast, colon, placenta, and dermal connective tissues was also observed. All other tissues, including brain, breast epithelium, colon epithelium, heart, intestine, kidney, liver, lung, skeletal muscle, pancreas, spleen, testis, thymus, thyroid, and uterus were negative for staining. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C for 20 minutes for antigen retrieval.
|Tested species reactivity||Bovine, Human, Mouse, Sheep, Rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Collagen type II from adult human knee cartilage and bovine nasal cartilage.|
|Purification||Antigen affinity chromatography|
|Storage buffer||BBS, pH 8.0, with 5mM EDTA|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:200|
|Western Blot (WB)||1:5000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26206 detects Collagen II from bovine and human samples. PA1-26206 is expected to cross react with most mammals due to sequence homology. This antibody shows negligable cross reactivity with the Collagens 1, 3, 4, 5, and 6.
PA1-26206 has been successfully used in ELISA, Immunohistochemistry (P), immunoprecipitation, and Western blot procedures.
The PA1-26206 immunogen is Collagen type II from adult human knee cartilage and bovine nasal cartilage.
Store product at 4°C short term and -20°C after adding 50% glycerol.
This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.