Immunohistochemical analysis of Collagen II in formalin-fixed, paraffin-embedded human bronchiolar cartilage using a Collagen II polyclonal antibody (Product # PA1-28531) at a dilution of 10 µg/mL for 1 hr at room temperature. Antigen retrieval was performed using a 0.01 M sodium citrate buffer, pH 6.0, at 99-100°C for 20 minutes. Secondary detection was performed using a Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at room temperature. Collagen II extracellular staining: precipitated red signal with hematoxylin purple nuclear counterstain.
|Tested species reactivity||Bovine, Human, Mammal|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Collagen Type II from adult human knee cartilage and bovine nasal cartilage|
|Purification||Antigen affinity chromatography|
|Storage buffer||BBS, pH 8.0, with 10mg/ml BSA, 5mM EDTA|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Frozen) (IHC (F))||1:200-1:1000|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:1000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:5000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28531 detects Collagen II from bovine, human samples.
PA1-28531 has been successfully used in immunohistochemistry (frozen tissue), immunoprecipitation, Western blot, and ELISA applications. The reactivity of the antibody is greatly diminished under denaturing and reducing conditions.
The PA1-28531 immunogen is Collagen Type II from adult human knee cartilage and bovine nasal cartilage
This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.