Immunohistochemical analysis of Collagen III in formalin-fixed, paraffin-embedded human skin (left, dermis) and testis (right) using a Collagen III polyclonal antibody (Product # PA1-28532) at a dilution of 1:400 for 45 min at room temperature. Slides were steamed in 0.01 M sodium citrate buffer, pH 6.0, at 99-100°C for 20 minutes for antigen retrieval.
|Tested species reactivity||Bovine, Human, Mammal|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Collagen Type III from adult human cartilage and bovine tissues|
|Purification||Antigen affinity chromatography|
|Storage buffer||BBS, pH 8.0, with 10mg/ml BSA, 5mM EDTA|
|Contains||0.01% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200-1:1000|
|Immunoprecipitation (IP)||Assay dependent|
|Western Blot (WB)||1:5000-1:10,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-28532 detects Collagen III from bovine, human samples.
PA1-28532 has been successfully used in immunohistochemistry (frozen tissue), immunoprecipitation, Western blot applications.
The PA1-28532 immunogen is: Collagen Type III from adult human cartilage and bovine tissues
This gene encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene. [provided by R. Dalgleish]
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