|Tested species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Full length, native protein extracted and purified from mouse tumor tissues.|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:500|
|Radioimmune Assays (RIA)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA1-26148 detects Collagen IV from mouse samples. This antibody does not react with mouse collagen types I and III, human collagen types IV and V, mouse fibronectin or mouse laminin.
PA1-26148 has been successfully used in radioimmune assay, Immunofluorescence, Immunohistochemistry (paraffin) and ELISA procedures.
The PA1-26148 immunogen is full length, native protein extracted and purified from mouse tumor tissues.
Collagen IV is a magor consitiuent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.