Invitrogen

Collagen IV Polyclonal Antibody

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:500

Immunocytochemistry (ICC)

1:80

Immunofluorescence (IF)

1:80

ELISA (ELISA)

1:200

Radioimmune Assays (RIA)

Assay Dependent

Product Specifications

Species Reactivity

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length, native protein extracted and purified from mouse tumor tissues.

Conjugate

Unconjugated

Form

Liquid

Concentration

3 mg/mL

Purification

Ion-exchange chromatography

Contains

no preservative

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_10977210

Product Specific Information

This antibody does not react with mouse collagen types I and III, human collagen types IV and V, mouse fibronectin or mouse laminin.

Target Information

Collagen IV is a major constituent of the basement membranes along with laminins and enactins. Collagen IV is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. Collagen IV can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: alpha1(IV) collagen; Arresten; Canstatin; col4a3; col4a4; Collagen 4; Collagen alpha-1(IV) chain; Collagen alpha-2(IV) chain; Collagen alpha-3(IV) chain; Collagen alpha-4(IV) chain; collagen type IV alpha3 chain; Del(8)Bru44H; procollagen, type IV, alpha 1; procollagen, type IV, alpha 2; procollagen, type IV, alpha 3; procollagen, type IV, alpha 4; retinal anterior wiring; Tumstatin; Type IV Collagen

Gene Aliases: [a]3(IV); [a]4(IV); alpha3(IV); Bru; Col4a-1; Col4a-2; Col4a1; Col4a2; Col4a3; Col4a4; Del(8)44H; E130010M05Rik; Raw; Svc

UniProt ID: (Mouse) P02463, (Mouse) P08122, (Mouse) Q9QZS0, (Mouse) Q9QZR9

Entrez Gene ID: (Mouse) 12826, (Mouse) 12827, (Mouse) 12828, (Mouse) 12829

Function(s)