|Tested species reactivity||Mouse, Non-human primate|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Collagen IV purified from murine EHS tumour.|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||4°C or -20°C if preferred|
|Tested Applications||Dilution *|
|ELISA (ELISA)||1:100 - 1:400|
|Immunohistochemistry (Paraffin) (IHC (P))||1:250-1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Collagen IV is a magor consitiuent of the basement membranes along with laminins and enactins. It is composed of alpha 1 IV chain and alpha 2 IV chain in 2:1 ratio. It can form insoluble fibers with high tensile strength. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.