Invitrogen

Complement C3 Polyclonal Antibody

Product Image Gallery

Complement C3 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of C3 in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min, using a C3 polyclonal antibody (Product # PA5-21349). Green: Primary antibody at a dilution of 1:200. Blue: Hoechst 33342 staining.

Complement C3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of C3 in paraffin-embedded mouse brain tissue using a polyclonal C3 antibody, C-term (Product # PA5-21349) at a dilution of 1:500.

Complement C3 Antibody in Western Blot (WB) — Western blot analysis of C3 using A) 30 µg 293T whole cell extract and B) 30 µg whole cell extract of human C3-transfected 293T cells. Samples were loaded onto a 5% SDS-PAGE gel and probed with a C3 polyclonal antibody (Product # PA5-21349) at a dilution of 1:20000.

Complement C3 Antibody in Immunoprecipitation (IP) — Immunoprecipitation and Western blot of C3 protein from HepG2 whole cell extracts using 5 µg of C3 antibody (Product # PA5-21349).

Product Details

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC)

1:100-1:1000

Immunofluorescence (IF)

1:100-1:1000

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1000

Immunoprecipitation (IP)

1:100-1:500

Western Blot (WB)

1:500-1:3000

Product Specifications

Species Reactivity

Human, Mouse

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fragment corresponding to a region within amino acids 1448 and 1655 of (Uniprot ID#P01024)

Conjugate

Unconjugated

Form

Liquid

Concentration

0.08 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11153785

Product Specific Information

Recommended positive controls: Huh7, HepG2.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

C3 is a key component of the complement system since classical and alternative activation pathways merge at the C3 activation step when C3 is split into C3a and C3b. The molecular mass of C3 is 185 kDa and it consists of two chains (110 kDa and 75 kDa) held together by disulfide bonds.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Acylation stimulating protein; acylation-stimulating protein cleavage product; AHUS5; ARMD9; ASP; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1; C3-beta-c; C3a anaphylatoxin; C3adesArg; C3bc; Complement C3; Complement C3 alpha chain; Complement C3 beta chain; Complement C3b alpha' chain; Complement C3c alpha' chain fragment 1; Complement C3c alpha' chain fragment 2; Complement C3d fragment; Complement C3dg fragment; Complement C3f fragment; Complement C3g fragment; complement component C3; complement component C3a; complement component C3b; CPAMD1; epididymis secretory sperm binding protein Li 62p; HSE-MSF; prepro-C3

Gene Aliases: AHUS5; AI255234; ARMD9; ASP; C3; C3a; C3b; CPAMD1; HEL-S-62p; HSE-MSF; Plp

UniProt ID: (Human) P01024, (Mouse) P01027

Entrez Gene ID: (Human) 718, (Mouse) 12266

Molecular Function: complement component cytokine defense/immunity protein enzyme modulator protease inhibitor serine protease inhibitor signaling molecule