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          • Primary Antibodies ›
          • Factor D Antibodies

          Invitrogen

          Complement Factor D Polyclonal Antibody

          View all (22) Factor D antibodies

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          Cite Complement Factor D Polyclonal Antibody

          Additional Information:

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          • Antibody Testing Data (1)
          Complement Factor D Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Complement Factor D Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 1

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          Complement Factor D Antibody (PA5-96941) in WB

          Western blot analysis of Complement Factor D in extracts of various cell lines. Samples were incubated with Complement Factor D Polyclonal antibody (Product # PA5-96941) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 20s. {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Complement Factor D Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Complement Factor D Polyclonal Antibody

          Product Details

          PA5-96941

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          ELISA (ELISA)

          1 µg/mL
          -
          Product Specifications

          Species Reactivity

          Human, Mouse

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          Recombinant fusion protein containing a sequence corresponding to amino acids 26-253 of human CFD (NP_0019192)
          3D Epitope / Immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          2.41 mg/mL

          Amount

          241 µg

          Purification

          Affinity Chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2808743

          Product Specific Information

          Immunogen sequence: ILGGREAEAH ARPYMASVQL NGAHLCGGVL VAEQWVLSAA HCLEDAADGK VQVLLGAHSL SQPEPSKRLY DVLRAVPHPD SQPDTIDHDL LLLQLSEKAT LGPAVRPLPW QRVDRDVAPG TLCDVAGWGI VNHAGRRPDS LQHVLLPVLD RATCNRRTHH DGAITERLMC AESNRRDSCK GDSGGPLVCG GVLEGVVTSG SRVCGNRKKP GIYTRVASYA AWIDSVLA; Positive Samples: A-549, 22Rv1, THP-1, Mouse lung

          Target Information

          Complement factor D is a serine protease of the alternative pathway of complement activation. The protein is a single chain polypeptide with a molecular mass of 25 kDa. Factor D cleaves factor B bound to C3b, generating the alternative pathway C3 convertase C3bBb and releasing the Ba fragment.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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          Cite this product

          Bioinformatics

          Protein Aliases: 28 kDa adipocyte protein; 28 Kda protein; C3 convertase activator; complement factor D (adipsin); complement factor D preproprotein; D component (adipsin) of complement; D component of complement (adipsin); DF; EC 3.4.21.46; properdin factor D; unnamed protein product

          View more View less

          Gene Aliases: ADIPSIN; ADN; DF; PFD

          View more View less

          UniProt ID: (Human) P00746, (Mouse) P03953

          View more View less

          Entrez Gene ID: (Human) 1675, (Mouse) 11537

          View more View less

          Function(s)
          serine-type endopeptidase activity serine-type peptidase activity endopeptidase activity
          Process(es)
          proteolysis complement activation complement activation, alternative pathway zymogen activation protein maturation Notch signaling pathway response to bacterium vascular smooth muscle cell differentiation retina development in camera-type eye
          It has to be done as per old AB suggested Products section.

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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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