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Invitrogen
Connexin 30.2 Polyclonal Antibody
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FIGURE: 1 / 7
Connexin 30.2 Antibody (40-7400) in IHC (P)
Immunohistochemistry analysis of Connexin 30.2 showing staining in the membrane and cytoplasm of paraffin-embedded human heart tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at ... View More
Product Details
40-7400
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide derived from the c-term region of the mouse connexin 30.2 protein
Conjugate
Form
Liquid
Concentration
0.25 mg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
0.1% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
40-7400 was used in the western blot analysis to successfully detect Connexin 30.2 in HeLa cells transfected with Cx30.2 and in immunofluorescence (IHC) analysis to successfully detect Connexin 30.2 in mouse heart sections.
Target Information
Gap junctions are responsible for transport of ions and metabolites between adjacent cells. These structures are made of two hemichannels, each formed by six connexin molecules. Connexin 30, coded by Cjb6 gene, is a member of the connexin family and is highly expressed in brain and skin. Immunohistochemically connexin 30 was localized in astrocytes, at gap junctions between these cells and on the astrocyte side of gap junctions between astrocytes and oligodendrocytes. Co-localization with connexin 43 was also observed in this study. Cytoskeleton, especially actin filaments, are important components in the processes of assembly, trafficking and stabilization of connexin 30 gap junctions. Knock-out mice studies have also demonstrated that connexin 30 deficiency impairs renal tubular ATP release and pressure natriuresis highlighting the importance of this molecule in kidney. Further, in vitro studies have also demonstrated that connexin 30 overexpression enhances cell proliferation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: connexin 30.2; Connexin-30.2; Connexin-31.9; Cx30.2; Cx31.9; Gap junction alpha-11 protein; Gap junction chi-1 protein; Gap junction delta-3 protein; gap junction membrane channel protein chi 1; gap junction protein, delta 3, 31.9kDa; unnamed protein product
Gene Aliases: Cx30.2; CX31.9; GJA11; GJC1; GJD3
UniProt ID: (Human) Q8N144, (Mouse) Q91YD1
Entrez Gene ID: (Human) 125111, (Mouse) 353155
cell communication
cell-cell signaling
response to glucose
negative regulation of heart rate
gap junction assembly
ion transmembrane transport
transmembrane transport
AV node cell to bundle of His cell communication by electrical coupling
cell communication involved in cardiac conduction
negative regulation of cell communication by electrical coupling involved in cardiac conduction
negative regulation of cardiac conduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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