40-7400 was used in the western blot analysis to successfully detect Connexin 30.2 in HeLa cells transfected with Cx30.2 and in immunofluorescence (IHC) analysis to successfully detect Connexin 30.2 in mouse heart sections.
Gap junctions are responsible for transport of ions and metabolites between adjacent cells. These structures are made of two hemichannels, each formed by six connexin molecules. Connexin 30, coded by Cjb6 gene, is a member of the connexin family and is highly expressed in brain and skin. Immunohistochemically connexin 30 was localized in astrocytes, at gap junctions between these cells and on the astrocyte side of gap junctions between astrocytes and oligodendrocytes. Co-localization with connexin 43 was also observed in this study. Cytoskeleton, especially actin filaments, are important components in the processes of assembly, trafficking and stabilization of connexin 30 gap junctions. Knock-out mice studies have also demonstrated that connexin 30 deficiency impairs renal tubular ATP release and pressure natriuresis highlighting the importance of this molecule in kidney. Further, in vitro studies have also demonstrated that connexin 30 overexpression enhances cell proliferation.
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Protein Aliases: connexin 30.2; connexin 31.9; Connexin-30.2; Connexin-31.9; Cx30.2; Cx31.9; Gap junction alpha-11 protein; Gap junction chi-1 protein; Gap junction delta-3 protein; gap junction membrane channel protein chi 1; gap junction protein, delta 3, 31.9kDa
Gene Aliases: Cx30.2; CX31.9; GJA11; GJC1; GJD3
Molecular Function: gap junction