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Immunofluorescence analysis of connexin 32 was performed on sections of adult mouse liver. Tissue sections on slides were probed for 24 h at 4°C in a humidified chamber with rabbit polyclonal anti-Cx32 (Cat. No. 34-5700), at an antibody concentration of 1-2 µg/ml diluted in 50 mM Tris-HCl, pH 7.4, containing 1.5% NaCl, 0.3% Triton X-100 (TBST) and 4% normal goat serum. After overnight incubation, sections were washed extensively for 1 h in TBST, and detection of primary antibody was performed for 1.5 h at room temperature with AlexaFluor-488-conjugated donkey anti-rabbit diluted 1:600 in TBST. Sections were then was in TBST, then in TBS (without triton) and then coversliped with anti-fade medium. Images were taken on a Zeiss Z2 scanning microscope at x40 objective magnification, and show immunofluorescence labelling of Cx32 localized at gap junctions between liver hepatocytes. Data courtesy of Dr. James Nagy's lab.
|Tested species reactivity||Mouse|
|Published species reactivity||Rat , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminal sequence of the human connexin 32 protein, downstream from the sequence identified by another Invitrogen Connexin 32 antibody, Cat. No. 71-0600.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|Immunohistochemistry (IHC)||1-2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:10-1:100|
|Western Blot (WB)||Assay Dependent|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
34-5700 was used in the immunofluorescence analysis to successfully detect Connexin 32 in gap junctions from mouse liver samples.
This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Re-evaluation of connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus.
34-5700 was used in immunocytochemistry to characterize connexins associated with motoneurons in rodent spinal cord, sexually dimorphic motor nuclei and trigeminal motor nucleus
|Bautista W,Rash JE,Vanderpool KG,Yasumura T,Nagy JI||The European journal of neuroscience (39:757)||2014|
Functional heterotypic interactions between astrocyte and oligodendrocyte connexins.
||Magnotti LM,Goodenough DA,Paul DL||Glia (59:26)||2011|
Inhibition of Mist1 homodimer formation induces pancreatic acinar-to-ductal metaplasia.
||Zhu L,Tran T,Rukstalis JM,Sun P,Damsz B,Konieczny SF||Molecular and cellular biology (24:2673)||2004|