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Immunofluorescence analysis of connexin 32 was performed on sections of adult mouse liver. Tissue sections on slides were probed for 24 h at 4°C in a humidified chamber with rabbit polyclonal anti-Cx32 (Cat. No. 71-0600), at an antibody concentration of 1-2 µg/ml diluted in 50 mM Tris-HCl, pH 7.4, containing 1.5% NaCl, 0.3% Triton X-100 (TBST) and 4% normal goat serum. After overnight incubation, sections were washed extensively for 1 h in TBST, and detection of primary antibody was performed for 1.5 h at room temperature with AlexaFluor-488-conjugated donkey anti-rabbit diluted 1:600 in TBST. Sections were then was in TBST, then in TBS (without triton) and then coversliped with anti-fade medium. Images were taken on a Zeiss Z2 scanning microscope at x40 objective magnification, and show immunofluorescence labelling of Cx32 localized at gap junctions between liver hepatocytes. Data courtesy of Dr. James Nagy's lab.
|Tested species reactivity||Human , Mouse , Rat|
|Published species reactivity||Rat , Human , Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Peptide corresponding to a portion of the cytoplasmic loop of rat connexin 32.|
|Storage buffer||PBS, pH 7.4|
|Contains||0.1% sodium azide|
|Tested Applications||Dilution *|
|ELISA (ELISA)||Assay Dependent|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (IHC)||1-2 µg/ml|
|Immunohistochemistry (Frozen) (IHC (F))||Assay Dependent|
|Western Blot (WB)||2 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
71-0600 was used in the immunofluorescence (IHC) and western blot analysis to successfully detect Connexin 32 in mouse liver.
This gene encodes a member of the gap junction protein family. The gap junction proteins are membrane-spanning proteins that assemble to form gap junction channels that facilitate the transfer of ions and small molecules between cells. According to sequence similarities at the nucleotide and amino acid levels, the gap junction proteins are divided into two categories, alpha and beta. Mutations in this gene cause X-linked Charcot-Marie-Tooth disease, an inherited peripheral neuropathy. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Absence of venous valves in mice lacking Connexin37.
71-0600 was used in immunohistochemistry to investigate the role of Cx37 during vasculogenesis in mice
|Munger SJ,Kanady JD,Simon AM||Developmental biology (373:338)||2013|
Connexin immunoreactivity in glial cells of the rat retina.
71-0600 was used in immunohistochemistry to study connexins in retinal glial cells.
|Zahs KR,Kofuji P,Meier C,Dermietzel R||The Journal of comparative neurology (455:531)||2003|
Localization of connexin26 and connexin32 in putative CO(2)-chemosensitive brainstem regions in rat.
||Solomon IC,Halat TJ,El-Maghrabi MR,O'Neal MH||Respiration physiology (129:101)||2001|
Impaired trafficking of connexins in androgen-independent human prostate cancer cell lines and its mitigation by alpha-catenin.
||Govindarajan R,Zhao S,Song XH,Guo RJ,Wheelock M,Johnson KR,Mehta PP||The Journal of biological chemistry (277:50087)||2002|
Role of gap junctions in fluid secretion of lacrimal glands.
||Walcott B,Moore LC,Birzgalis A,Claros N,Valiunas V,Ott T,Willecke K,Brink PR||American journal of physiology. Cell physiology (282:C501)||2002|
Connexin 26 expression and extensive gap junctional coupling in cultures of GT1-7 cells secreting gonadotropin-releasing hormone.
71-0600 was used in immunocytochemistry and western blot to assess gap junction connectivity.
|Hu L,Olson AJ,Weiner RI,Goldsmith PC||Neuroendocrinology (70:221)||1999|
The coiled-coil domain of occludin can act to organize structural and functional elements of the epithelial tight junction.
||Nusrat A,Chen JA,Foley CS,Liang TW,Tom J,Cromwell M,Quan C,Mrsny RJ||The Journal of biological chemistry (275:29816)||2000|
Immunogold evidence that neuronal gap junctions in adult rat brain and spinal cord contain connexin-36 but not connexin-32 or connexin-43.
||Rash JE,Staines WA,Yasumura T,Patel D,Furman CS,Stelmack GL,Nagy JI||Proceedings of the National Academy of Sciences of the United States of America (97:7573)||2000|
Intracellular transport, assembly, and degradation of wild-type and disease-linked mutant gap junction proteins.
71-0600 was used in western blot to investigate the intracellular transport, assembly, and degradation of three Charcot-Marie-Tooth disease-linked connexin32 mutants stably expressed in PC2 cells.
|VanSlyke JK,Deschenes SM,Musil LS||Molecular biology of the cell (11:1933)||2000|
Cx32, CMTX1, Cnx32, CX32, CMTX
GAP junction 28 kDa liver protein, connexin 32, connexin-32, gap junction beta-1 protein, gap junction membrane channel protein beta 1, Gap junction beta-1 protein, Cx32