Invitrogen

Connexin 37 Polyclonal Antibody

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Connexin 37 Antibody in Immunohistochemistry (IHC) — Immunofluorescence analysis of Connexin 37 (Cx37) was performed on sections of adult mouse heart. Tissue sections on slides were probed for 24 h at 4°C in a humidified chamber with rabbit polyclonal anti-Cx37 (Product # 40-4200) at an antibody concentration of 1-2 µg/mL diluted in 50 mM Tris-HCl, pH 7.4, containing 1.5% NaCl, 0.3% Triton X-100 (TBST) and 4% normal goat serum. After overnight incubation, sections were washed extensively for 1 h in TBST, and detection of primary antibody was performed for 1.5 h at room temperature with AlexaFluor-488-conjugated donkey anti-rabbit diluted 1:600 in TBST. Sections were then washed in TBST, then in TBS (without triton) and then coversliped with anti-fade medium. Images were taken on a Zeiss 710 confocal microscope at x40 objective magnification, and show immunofluorescence labelling of Cx37 distributed at presumptive gap junctions along a blood vessel in adult mouse heart. Data courtesy of Dr. James Nagy's lab.

Connexin 37 Antibody in Western Blot (WB) — Western blot analysis of Connexin 37 was performed by loading 20 µg of Mouse Skeletal Muscle tissue lysate using Novex®NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX), XCell SureLock Electrophoresis System (Product # EI0002), Novex® Sharp Pre-Stained Protein Standard (LC5800), and iBlot® Dry Blotting System (IB21001). Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk for 1 hour at room temperature. Connexin 37 was detected at ~ 37 kDa using Connexin 37 Rabbit Polyclonal Antibody (Product # 40-4200) at 1-3 µg/mL in 5% skim milk at 4°C overnight on a rocking platform. Goat Anti-Rabbit IgG - HRP Secondary Antibody (G21234) at 1:5000 dilution was used and chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

Connexin 37 Antibody in Immunohistochemistry (Frozen) (IHC (F)) — Published figure using Connexin 37 polyclonal antibody (Product # 40-4200) in Immunohistochemistry (Frozen)

Product Details

40-4200

Applications	Tested Dilution	Publications
Western Blot (WB)	1-3 µg/mL	View 2 publications 2 publications
Immunohistochemistry (IHC)	-	View 2 publications 2 publications
Immunohistochemistry (Frozen) (IHC (F))	Assay-dependent	View 1 publication 1 publication

Product Specifications
Species Reactivity	Mouse
Published species	Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Synthetic peptide derived from the C-terminal region of the mouse connexin 37 protein View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.25 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.4
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2533464

Product Specific Information

40-4200 was used in the immunofluorescence (IHC) analysis to successfully detect Connexin 37 in mouse heart sections.

Target Information

This gene encodes a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene have been associated with atherosclerosis and a higher risk of myocardial infarction.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

GATA2 controls lymphatic endothelial cell junctional integrity and lymphovenous valve morphogenesis through miR-126.

Development (Cambridge, England)

Mahamud MR,Geng X,Ho YC,Cha B,Kim Y,Ma J,Chen L,Myers G,Camper S,Mustacich D,Witte M,Choi D,Hong YK,Chen H,Varshney G,Engel JD,Wang S,Kim TH,Lim KC,Srinivasan RS

40-4200 was used in Immunohistochemistry to define a new mechanism of GATA2 activity and uncover miR-126 as a novel regulator of mammalian lymphatic vascular development.

Tue Nov 05 00:00:00 EST 2019

Lymph flow regulates collecting lymphatic vessel maturation in vivo.

The Journal of clinical investigation

Sweet DT,Jiménez JM,Chang J,Hess PR,Mericko-Ishizuka P,Fu J,Xia L,Davies PF,Kahn ML

Published figure using Connexin 37 polyclonal antibody (Product # 40-4200) in Western Blot

Mon Aug 03 00:00:00 EDT 2015

Connexin37 and Connexin43 deficiencies in mice disrupt lymphatic valve development and result in lymphatic disorders including lymphedema and chylothorax.

Developmental biology

Kanady JD,Dellinger MT,Munger SJ,Witte MH,Simon AM

40-4200 was used in Immunohistochemistry to show that at least three Cxs are expressed in the developing lymphatic vasculature and, when defective, are associated with clinically manifest lymphatic disorders in mice and man.