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Cortactin Antibody (PA5-120325) in IHC (P)
Immunohistochemistry analysis of Cortactin in paraffin-embedded human mammary cancer. Samples were incubated with Cortactin Polyclonal antibody (Product # PA5-120325) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
PA5-120325
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Cortactin (NP_612632.1).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.91 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2913897
Product Specific Information
Positive test controls include: MCF7, HeLa. The target is usually found in the following locations: Cell junction, Cell membrane, Cell projection, Cytoplasm, Cytoplasmic side, Membrane, Peripheral membrane protein, cell cortex, clathrin-coated pit, cytoskeleton, dendrite, dendritic spine, focal adhesion, lamellipodium, podosome, ruffle.
Immunogen sequence: MWKASAGHAV SIAQDDAGAD DWETDPDFVN DVSEKEQRWG AKTVQGSGHQ EHINIHKLRE NVFQEHQTLK EKELETGPKA SHGYGGKFGV EQDRMDKSAV GHEYQSKLSK HCSQVDSVRG FGGKFGVQMD RVDQSAVGFE YQGKTEKHAS QKDYSSGFGG KYGVQADRVD KSAVGFDYQG KTEKHESQRD YSKGFGGKYG
Target Information
Cortactin (Cttn) is a ubiquitous actin-binding protein that was originally identified as a substrate for Src. It contributes to the organization of the actin cytoskeleton and cell shape. Cortactin also plays a role in the formation of lamellipodia and cell migration. It is accumulated in peripheral, actin-enriched structures of cells suggesting that cortactin facilitates actin network formation. Cortactin has four major domains of interest the N-terminal acidic (NTA) and tandem repeats domains and the C-terminal proline-rich and SH3 Domains. NTA associates with the Arp2/3 and WASP complex at F-actin branches. Cortactin is involved in promoting cell motility and invasion, including a critical role in invadopodia, actin rich-subcellular protrusions associated with degradation of the ECM by cancer cells. Cortactin is phosphorylated by src family kinases at Y421, Y466, and Y482 and S405 and S418 that are phosphorylated by Erk family kinases.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 1110020L01Rik; Amplaxin; ems1 sequence (mammary tumor and squamous cell carcinoma-associated (p80/85 src substrate); FLJ34459; mammary tumor and squamous cell carcinoma associated (p80/85 src substrate); Oncogene EMS1; Oncogene SRC8; Src substrate cortactin; src8
Gene Aliases: 1110020L01Rik; CTTN; Cttnb; EMS1
UniProt ID: (Human) Q14247, (Mouse) Q60598, (Rat) Q66HL2
Entrez Gene ID: (Human) 2017, (Mouse) 13043, (Rat) 60465
intracellular protein transport
receptor-mediated endocytosis
substrate-dependent cell migration, cell extension
actin filament polymerization
regulation of axon extension
positive regulation of actin filament polymerization
actin cytoskeleton reorganization
positive regulation of smooth muscle contraction
focal adhesion assembly
neuron projection morphogenesis
cell motility
dendritic spine maintenance
lamellipodium organization
cell-cell adhesion
regulation of mitophagy
negative regulation of extrinsic apoptotic signaling pathway
endocytosis
brain development
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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