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Invitrogen
Cripto Polyclonal Antibody
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FIGURE: 1 / 8
Cripto Antibody (PA5-116016) in ICC/IF
Immunocytochemistry analysis of Cripto in HepG2 cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-116016) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-116016
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human TDGF1(Accession P13385), corresponding to amino acid residues A96-L146.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Antibody detects endogenous levels of total TDGF1.
Target Information
Cripto (TDGF1) is a GPI-anchored cell membrane proetin involved in Nodal signaling. Cell associated TDGF1 acts as a a Nodeal coreceptor in cis. Shedding of TDGF1 by TMEM8A modulated Nodal signaling by allowing soluble TDGF1 to act as a Nodal coreceptor on other cells. TDGF1 is expressed both in ES cells and during the early phases of embryo development, while in adults it is reactivated in a wide range of epithelial cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CRGF; Cripto growth factor; Cripto, EGF-CFC family member; Cripto-1; Cripto-1 growth factor; Cripto-3; Epidermal growth factor-like Cripto protein; Epidermal growth factor-like cripto protein CR1; Protein Cripto; TDGF2; Teratocarcinoma-derived growth factor; Teratocarcinoma-derived growth factor 1; TERATOCARCINOMA-DERIVED GROWTH FACTOR PRECURSOR (EPIDERMAL GROWTH FACTOR-LIKE CRIPTO PROTEIN) (CRIPTO GROWTH FACTOR); unnamed protein product
Gene Aliases: CR; CR-1; CR1; CRGF; CRIPTO; CRIPTO-1; TDGF1
UniProt ID: (Human) P13385, (Mouse) P51865
Entrez Gene ID: (Human) 6997, (Rat) 680246, (Mouse) 21667
blood vessel development
morphogenesis of a branching structure
cell migration involved in sprouting angiogenesis
signal transduction
epidermal growth factor receptor signaling pathway
determination of left/right symmetry
heart development
positive regulation of cell proliferation
anterior/posterior axis specification, embryo
embryo development ending in birth or egg hatching
anterior/posterior pattern specification
regulation of signal transduction
positive regulation of endothelial cell migration
cell differentiation
positive regulation of cell migration
mammary gland development
cellular response to hepatocyte growth factor stimulus
nodal signaling pathway
negative regulation of apoptotic process
positive regulation of MAP kinase activity
positive regulation of MAPK cascade
cellular response to fibroblast growth factor stimulus
cellular response to interferon-gamma
cellular response to interleukin-6
cellular response to tumor necrosis factor
cellular response to epidermal growth factor stimulus
activation of MAPK activity
vasculogenesis
in utero embryonic development
positive regulation of cell-matrix adhesion
gastrulation
anterior/posterior axis specification
peptidyl-serine phosphorylation
regulation of cell migration
BMP signaling pathway
negative regulation of transforming growth factor beta receptor signaling pathway
positive regulation of transcription from RNA polymerase II promoter
positive regulation of fibroblast proliferation
positive regulation of peptidyl-tyrosine phosphorylation
cardiac muscle cell differentiation
canonical Wnt signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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