Invitrogen

Cullin 1 Polyclonal Antibody

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Cullin 1 Antibody in Immunohistochemistry (IHC) — Immunohistochemical analysis of Cullin-1 using anti-Cullin-1 Polyclonal Antibody (Product # PA5-32370) in Colon Carcinoma Cancer Tissue. The recommened dilution for this antibody in immunohistochemistry applications is 1:50.

Product Details

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide derived from the C-terminus of human cullin-1

Conjugate

Unconjugated

Form

Liquid

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

<0.1% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2549840

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is colon carcinoma or tonsil tissue.

Target Information

CUL1 gene ontology annotations related to this gene include animal organ morphogenesis; apoptotic process; cell proliferation; protein monoubiquitination; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CUL-1; Cullin-1

Gene Aliases: CUL1

UniProt ID: (Human) Q13616

Entrez Gene ID: (Human) 8454

Molecular Function: ligase ubiquitin-protein ligase

G1/S transition of mitotic cell cycle G2/M transition of mitotic cell cycle protein polyubiquitination stimulatory C-type lectin receptor signaling pathway protein monoubiquitination cell cycle arrest cell proliferation negative regulation of cell proliferation organ morphogenesis viral process Wnt signaling pathway protein ubiquitination SCF-dependent proteasomal ubiquitin-dependent protein catabolic process NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process T cell receptor signaling pathway stress-activated MAPK cascade positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition intrinsic apoptotic signaling pathway

Process(es)

ubiquitin-protein transferase activity protein binding ubiquitin protein ligase binding ubiquitin protein ligase activity