|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide derived from the C-terminus of human cullin-1|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 10 min at room temperature. A suggested positive control is colon carcinoma or tonsil tissue.
Cullin-1 is a member of the family of human cullin genes (cullin-1, 2, 3, 4a, 4b, and 5) homologous to the s. cerevisiae cdc53 gene. Cullin-1 forms a complex with human p19skp1 and F box protein p45skp2, both of which, together with cdc53, as three subunits, form an ubiquitin ligase controlling proteolysis of G1 cell cycle regulatory proteins. The cullin-1/p19skp1/p45skp2 complex is thought to play a role in the ubiquitination of G1 regulatory proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.