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Invitrogen
Cullin 5 Polyclonal Antibody
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FIGURE: 1 / 1
Cullin 5 Antibody (PA5-92425) in WB
Western blot analysis of Cullin 5 in rat heart. Samples were incubated with Cullin 5 Polyclonal antibody (Product # PA5-92425) using a dilution of 1:2,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
Product Details
PA5-92425
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CUL5 (NP_0034692)
Conjugate
Form
Liquid
Concentration
0.82 mg/mL
Amount
82 µg
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: MATSNLLKNK GSLQFEDKWD FMRPIVLKLL RQESVTKQQW FDLFSDVHAV CLWDDKGPAK IHQALKEDIL EFIKQAQARV LSHQDDTALL KAYIVEWRKF FTQCDILPKP FCQLEITLMG KQGSNKKSNV EDSIVRKLML DTWNESIFSN IKNRLQDSAM KLVHAERLGE AFDSQLVIGV RESYVNLCSN PEDKLQIYRD NFEKAYLDST ERFYRTQAPS YLQQNGVQNY MKYADAKLKE EEKRALRYLE TRRECNSVEA LMECCVNALV TSFKETILAE CQGMIKRNET EKLHLMFSLM; Positive Samples: 293T, PC-3, Mouse heart, Mouse gastrocnemius muscle
Target Information
CUL5 is a core component of multiple SCF-like ECS (Elongin-Cullin 2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. As a scaffold protein may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The functional specificity of the E3 ubiquitin-protein ligase complex depends on the variable substrate recognition component. ECS(SOCS1) seems to direct ubiquitination of JAk2. It seems to be involved poteosomal degradation of p53/TP53 stimulated by adenovirus E1B-55 kDa protein and may form a cell surface vasopressin receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CUL-5; Cullin-5; Cullin-5 (vasopressin-activated calcium-mobilizing receptor-1); SCF complex component; unnamed protein product; VACM-1; Vasopressin-activated calcium-mobilizing receptor 1; vasopressin-activated calcium-mobilizing receptor protein; Vasopressin-activated calcium-mobilizing receptor-1
Gene Aliases: CUL-5; CUL5; VACM-1; VACM1
UniProt ID: (Human) Q93034, (Rat) Q9JJ31
Entrez Gene ID: (Human) 8065, (Rat) 64624
ubiquitin-protein transferase activity
calcium channel activity
protein binding
protein binding, bridging
ubiquitin protein ligase binding
signaling receptor activity
ubiquitin ligase complex scaffold activity
vasopressin receptor activity
protein heterodimerization activity
ubiquitin-protein ligase
G1/S transition of mitotic cell cycle
epithelial to mesenchymal transition
ubiquitin-dependent protein catabolic process
signal transduction
proteasomal protein catabolic process
negative regulation of epithelial to mesenchymal transition
cell migration
protein ubiquitination
layer formation in cerebral cortex
positive regulation of cell migration
endoplasmic reticulum unfolded protein response
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
reelin-mediated signaling pathway
ERBB2 signaling pathway
erythropoietin-mediated signaling pathway
proteasome-mediated ubiquitin-dependent protein catabolic process
innate immune response
defense response to virus
positive regulation of focal adhesion assembly
negative regulation of focal adhesion assembly
symbiont-mediated suppression of host innate immune response
growth hormone receptor signaling pathway
JAK-STAT cascade involved in growth hormone signaling pathway
negative regulation of growth hormone receptor signaling pathway
calcium ion transmembrane transport
protein K48-linked ubiquitination
protein K11-linked ubiquitination
intrinsic apoptotic signaling pathway
negative regulation of focal adhesion disassembly
cGAS/STING signaling pathway
negative regulation of cGAS/STING signaling pathway
regulation of neuron migration
protein polyubiquitination
response to osmotic stress
G-protein coupled receptor signaling pathway
cerebral cortex radially oriented cell migration
radial glia guided migration of Purkinje cell
regulation of cytosolic calcium ion concentration
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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