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Invitrogen

Cyclin D1 Polyclonal Antibody

View all (109) Cyclin D1 antibodies

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Cyclin D1 Polyclonal Antibody

Product Details

PA5-85257

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

Assay-dependent
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-

ELISA (ELISA)

1:1,000-1:10,000
-

Immunoprecipitation (IP)

1:100-1:500
-
Product Specifications

Species Reactivity

Dog, Cat, Human, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Full length human Cyclin D1 Recombinant protein.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.21 mg/mL

Amount

121 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2792400

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Mouse (93%), Rat (93%), Xenopus laevis (80%), Dog (94%), Chicken (86%), Rhesus Monkey (99%), Bovine (93%).

Positive Control: HeLa, MCF-7, MDA-MB-231, Huh 7, H184B5F5/M10, BT-474, HCC1937, Hs578T, MDA-MB-231, MDA-MB-361, MDA-MB-453, T47D, ZR-75-1.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Cyclin D1 (PRAD-1, bcl-1) is one of the key cell cycle regulators, and functions in association with cdk4 and/or cdk6 by phosphorylating the Rb protein. Cyclin D1 is a putative proto-oncogene overexpressed in a wide variety of human neoplasms including mantle cell lymphomas (MCL). In addition, cyclin D1 positively regulates protein phosphorylation, mammary gland epithelial cell proliferation, and fat cell differentiation. In humans, the CCND1 gene encoding cyclin D1 is present on chromosome 11. Cyclin D1 is a cytoplasmic and nuclear protein that is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Without growth factor-mediated stimulation, Cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest. The Cyclin D1 protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The Cyclin D1 protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Cyclin D1 has been successfully employed and is a promising tool for further studies in both cell cycle biology and cancer associated abnormalities.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B-cell CLL/lymphoma 1; B-cell lymphoma 1 protein; BCL-1; BCL-1 oncogene; CCND1; CCND1 protein; cDNA; FLJ93625; G1/S-specific cyclin-D1; mRNA; parathyroid adenomatosis 1; PRAD-1; PRAD1 oncogene; unnamed protein product

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Gene Aliases: BCL1; CCND1; D11S287E; PRAD1; U21B31

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UniProt ID: (Dog) Q64HP0, (Human) P24385, (Rat) P39948

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Entrez Gene ID: (Dog) 449028, (Human) 595, (Rat) 58919

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Function(s)
transcription corepressor activity protein kinase activity protein binding kinase activity cyclin-dependent protein serine/threonine kinase regulator activity enzyme binding protein kinase binding histone deacetylase binding protein serine/threonine kinase activator activity macromolecular complex binding cyclin-dependent protein serine/threonine kinase activator activity proline-rich region binding transcription factor binding protein complex binding kinase activator
Process(es)
G1/S transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter positive regulation of protein phosphorylation cellular response to DNA damage stimulus positive regulation of G2/M transition of mitotic cell cycle mitotic G1 DNA damage checkpoint positive regulation of cyclin-dependent protein serine/threonine kinase activity cell division response to UV-A negative regulation of cell cycle arrest re-entry into mitotic cell cycle liver development lactation cell proliferation positive regulation of cell proliferation response to xenobiotic stimulus response to iron ion response to X-ray Wnt signaling pathway neuron differentiation negative regulation of epithelial cell differentiation endoplasmic reticulum unfolded protein response organ regeneration response to magnesium ion response to estradiol response to vitamin E Leydig cell differentiation mammary gland epithelial cell proliferation positive regulation of mammary gland epithelial cell proliferation negative regulation of neuron apoptotic process response to estrogen response to leptin fat cell differentiation response to ethanol response to steroid hormone response to glucocorticoid response to corticosterone response to calcium ion regulation of cell cycle mammary gland alveolus development liver regeneration positive regulation of G1/S transition of mitotic cell cycle positive regulation of mitotic cell cycle phase transition regulation of G1/S transition of mitotic cell cycle transcription, DNA-templated protein phosphorylation response to organic substance response to organonitrogen compound response to organic cyclic compound negative regulation of Wnt signaling pathway response to drug regulation of protein kinase activity canonical Wnt signaling pathway cellular response to organic substance
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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