Immunohistochemistry analysis of Cyclin E showing staining in the nucleus and weak cytoplasm of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Cyclin E Rabbit Polyclonal Antibody (PA516237) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||A synthetic peptide derived from the C-terminus of human cyclin E|
|Storage buffer||PBS, pH 7.4, with 0.2% BSA|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunofluorescence (IF)||Assay Dependent|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Immunoprecipitation (IP)||10 µg/ml|
|Western Blot (WB)||5-10 µg/mL|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Immunohistochemistry (IHC)||See 1 publications below|
PA5-16237 targets Cyclin E in IF, IP, and WB applications and shows reactivity with mouse and Human samples.
The PA5-16237 immunogen is a synthetic peptide derived from the C-terminus of human cyclin E.
Cyclin E is a regulatory subunit of cdk-2 and controls G1/S transition during the mammalian cell cycle. Multiple isoforms of cyclin E are expressed only in tumors but not in the normal tissues, suggesting a post-transcriptional regulation of cyclin E. In vitro analyses indicated that these truncated variant isoforms of cyclin E are able to phosphorylate histone H1. Alterations in cyclin E protein have been implicated as indicators of worse prognosis in various cancers.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Smoothened controls cyclin D2 expression and regulates the generation of intermediate progenitors in the developing cortex.
PA5-16237 was used in immunohistochemistry to study the modulation of cyclin D2 expression by Smoothened and its role in the generation of neocortical intermediate progenitors
|Komada M,Iguchi T,Takeda T,Ishibashi M,Sato M||Neuroscience letters (547:87)||2013|