Proteintech

Cytokeratin 8 Polyclonal Antibody

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Cytokeratin 8 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of MCF-7 cells using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Cytokeratin 8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10384-1-AP (Cytokeratin 8 antibody at dilution of 1:200 (under 40x lens).

Cytokeratin 8 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of ( 4% PFA) fixed human breast cancer tissue using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Cytokeratin 8 Antibody in Western Blot (WB) — Rat skin tissue were subjected to SDS PAGE followed by western blot with 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

Cytokeratin 8 Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2 ug Cytokeratin 8 antibody (10384-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) with dilution 1:1500.

Cytokeratin 8 Antibody in Immunoprecipitation (IP) — IP result of anti-Cytokeratin 8 (IP:10384-1-AP, 4ug; Detection:10384-1-AP 1:1000) with HeLa cells lysate 2000ug.

Product Details

10384-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:3,000	-
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:50-1:500	-
Immunocytochemistry (ICC/IF)	1:50-1:500	-
Flow Cytometry (Flow)	0.2 µg/1x10^6 cells	-
Immunoprecipitation (IP)	0.5-4.0 µg	-

Product Specifications
Species Reactivity	Human, Mouse, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Cytokeratin 8 Fusion Protein Ag0488 (1-483 aa encoded by BC000654) View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: MSIRVTQKS YKVSTSGPRA FSSRSYTSGP GSRISSSSFS RVGSSNFRGG LGGGYGGASG MGGITAVTVN QSLLSPLVLE VDPNIQAVRT QEKEQIKTLN NKFASFIDKV RFLEQQNKML ETKWSLLQQQ KTARSNMDNM FESYINNLRR QLETLGQEKL KLEAELGNMQ GLVEDFKNKY EDEINKRTEM ENEFVLIKKD VDEAYMNKVE LESRLEGLTD EINFLRQLYE EEIRELQSQI SDTSVVLSMD NSRSLDMDSI IAEVKAQYED IANRSRAEAE SMYQIKYEEL QSLAGKHGDD LRRTKTEISE MNRNISRLQA EIEGLKGQRA SLEAAIADAE QRGELAIKDA NAKLSELEAA LQRAKQDMAR QLREYQELMN VKLALDIEIA TYRKLLEGEE SRLESGMQNM SIHTKTTSGY AGGLSSAYGG LTSPGLSYSL GSSFGSGAGS SSFSRTSSSR AVVVKKIETR DGKLVSESSD VLPK (1-483 aa encoded by BC000654)

Target Information

Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: C CY K2C8; CK 8; CK-8; cytokeratin 8; Cytokeratin endo A; Cytokeratin-8; hard keratin; K-8; K8; keratin 8, type II; keratin complex 2, basic, gene 8; Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8

Gene Aliases: AA960620; AL022697; AU019895; CARD2; CK-8; CK8; CYK8; CYKER; EndoA; K2C8; K8; KO; Krt-2.8; Krt2-8; KRT8

UniProt ID: (Human) P05787, (Mouse) P11679, (Rat) Q10758

Entrez Gene ID: (Human) 3856, (Mouse) 16691, (Rat) 25626

Function(s)

cell morphogenesis involved in differentiation multicellular organism development tumor necrosis factor-mediated signaling pathway sarcomere organization response to hydrostatic pressure response to other organism cell differentiation involved in embryonic placenta development extrinsic apoptotic signaling pathway hepatocyte apoptotic process viral process

Process(es)

structural molecule activity protein binding protein complex binding scaffold protein binding