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Cytokeratin 8 Polyclonal Antibody

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Antibody Testing Data (24)

Cytokeratin 8 Antibody in Western Blot (WB)

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Cytokeratin 8 Antibody (10384-1-AP) in WB

Various lysates were subjected to SDS PAGE followed by western blot with (Product # 10384-1-AP) Cytokeratin 8 antibody at dilution of 1:30,000 incubated at room temperature for 1.5 hours.

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Cytokeratin 8 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Cytokeratin 8 antibody (Product # 10384-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Cytokeratin 8 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using (Product # 10384-1-AP) Cytokeratin 8 antibody at dilution of 1:2,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cytokeratin 8 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of ( 4% PFA) fixed human breast cancer tissue using 10384-1-AP (Cytokeratin 8 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Cytokeratin 8 Antibody in Flow Cytometry (Flow) — 1X10^6 MCF-7 cells were stained with 0.2 ug Cytokeratin 8 antibody (10384-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) with dilution 1:1500.

Cytokeratin 8 Antibody in Immunoprecipitation (IP) — IP result of anti-Cytokeratin 8 (IP:10384-1-AP, 4ug; Detection:10384-1-AP 1:1000) with HeLa cells lysate 2000ug.

Product Details

10384-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3,000

View 5 publications 5 publications

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:50-1:500

Immunocytochemistry (ICC/IF)

1:50-1:500

View 5 publications 5 publications

Flow Cytometry (Flow)

0.2 µg/1x10^6 cells

Immunoprecipitation (IP)

0.5-4.0 µg

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Not Applicable

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Cytokeratin 8 Fusion Protein Ag0488 (1-483 aa encoded by BC000654)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: MSIRVTQKS YKVSTSGPRA FSSRSYTSGP GSRISSSSFS RVGSSNFRGG LGGGYGGASG MGGITAVTVN QSLLSPLVLE VDPNIQAVRT QEKEQIKTLN NKFASFIDKV RFLEQQNKML ETKWSLLQQQ KTARSNMDNM FESYINNLRR QLETLGQEKL KLEAELGNMQ GLVEDFKNKY EDEINKRTEM ENEFVLIKKD VDEAYMNKVE LESRLEGLTD EINFLRQLYE EEIRELQSQI SDTSVVLSMD NSRSLDMDSI IAEVKAQYED IANRSRAEAE SMYQIKYEEL QSLAGKHGDD LRRTKTEISE MNRNISRLQA EIEGLKGQRA SLEAAIADAE QRGELAIKDA NAKLSELEAA LQRAKQDMAR QLREYQELMN VKLALDIEIA TYRKLLEGEE SRLESGMQNM SIHTKTTSGY AGGLSSAYGG LTSPGLSYSL GSSFGSGAGS SSFSRTSSSR AVVVKKIETR DGKLVSESSD VLPK (1-483 aa encoded by BC000654)

Target Information

Cytokeratin 8 is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. Cutokeratin 8 typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

COVID-19 lung disease shares driver AT2 cytopathic features with Idiopathic pulmonary fibrosis.

bioRxiv : the preprint server for biology

Sinha S,Castillo V,Espinoza CR,Tindle C,Fonseca AG,Dan JM,Katkar GD,Das S,Sahoo D,Ghosh P

Mon Jul 18 00:00:00 EDT 2022

D-tagatose protects against oleic acid-induced acute respiratory distress syndrome in rats by activating PTEN/PI3K/AKT pathway.

Frontiers in immunology

Huang J,Wang B,Tao S,Hu Y,Wang N,Zhang Q,Wang C,Chen C,Gao B,Cheng X,Li Y

Tue Oct 04 00:00:00 EDT 2022

D-tagatose protects against oleic acid-induced acute respiratory distress syndrome in rats by activating PTEN/PI3K/AKT pathway.

Frontiers in immunology

Huang J,Wang B,Tao S,Hu Y,Wang N,Zhang Q,Wang C,Chen C,Gao B,Cheng X,Li Y

Tue Oct 04 00:00:00 EDT 2022

COVID-19 lung disease shares driver AT2 cytopathic features with Idiopathic pulmonary fibrosis.

EBioMedicine

Sinha S,Castillo V,Espinoza CR,Tindle C,Fonseca AG,Dan JM,Katkar GD,Das S,Sahoo D,Ghosh P

Mon Aug 01 00:00:00 EDT 2022

Comparative study of the external auditory canal in humans and large mammals.

Anatomical record (Hoboken, N.J. : 2007)

Wang J,He A,Yin D,Zhu Y,Zhou G,Zhang T

Tue Feb 01 00:00:00 EST 2022

The helix-loop-helix transcriptional regulator Id4 is required for terminal differentiation of luminal epithelial cells in the prostate.

Oncoscience

Hewa Bostanthirige D,Komaragiri SK,Joshi JB,Alzahrani M,Saini I,Jain S,Bowen NJ,Havrda MC,Chaudhary J

Sat Apr 24 00:00:00 EDT 2021

ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.

Frontiers in cell and developmental biology

Yu S,Zhang R,Shen Q,Zhu Z,Zhang J,Wu X,Zhao W,Li N,Yang F,Wei H,Hua J

Fri Oct 08 00:00:00 EDT 2021

ESRRB Facilitates the Conversion of Trophoblast-Like Stem Cells From Induced Pluripotent Stem Cells by Directly Regulating CDX2.

Frontiers in cell and developmental biology

Yu S,Zhang R,Shen Q,Zhu Z,Zhang J,Wu X,Zhao W,Li N,Yang F,Wei H,Hua J

Fri Oct 08 00:00:00 EDT 2021

SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network.

Journal of cell science

Chakraborty A,Lin WC,Lin YT,Huang KJ,Wang PY,Chang IY,Wang HI,Ma KT,Wang CY,Huang XR,Lee YH,Chen BC,Hsieh YJ,Chien KY,Lin TY,Liu JL,Sung LY,Yu JS,Chang YS,Pai LM

Mon May 11 00:00:00 EDT 2020

SNAP29 mediates the assembly of histidine-induced CTP synthase filaments in proximity to the cytokeratin network.

Journal of cell science

Chakraborty A,Lin WC,Lin YT,Huang KJ,Wang PY,Chang IY,Wang HI,Ma KT,Wang CY,Huang XR,Lee YH,Chen BC,Hsieh YJ,Chien KY,Lin TY,Liu JL,Sung LY,Yu JS,Chang YS,Pai LM

Mon May 11 00:00:00 EDT 2020

Alternative promotion and suppression of metastasis by JNK2 governed by its phosphorylation.

Oncotarget

Hu S,Dong X,Gao W,Stupack D,Liu Y,Xiang R,Li N

Tue Aug 22 00:00:00 EDT 2017

Alternative promotion and suppression of metastasis by JNK2 governed by its phosphorylation.

Oncotarget

Hu S,Dong X,Gao W,Stupack D,Liu Y,Xiang R,Li N

Tue Aug 22 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: CK-8; cytokeratin 8; Cytokeratin endo A; Cytokeratin-8; K8; keratin 8, type II; keratin complex 2, basic, gene 8; Keratin, type II cytoskeletal 8; Keratin-8; Type-II keratin Kb8

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Gene Aliases: AA960620; AL022697; AU019895; CARD2; CK-8; CK8; CYK8; CYKER; EndoA; K2C8; K8; KO; Krt-2.8; Krt2-8; KRT8

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UniProt ID: (Human) P05787, (Mouse) P11679, (Rat) Q10758

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Entrez Gene ID: (Human) 3856, (Mouse) 16691, (Rat) 25626

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Function(s)

cell morphogenesis involved in differentiation multicellular organism development tumor necrosis factor-mediated signaling pathway sarcomere organization response to hydrostatic pressure response to other organism cell differentiation involved in embryonic placenta development extrinsic apoptotic signaling pathway hepatocyte apoptotic process viral process

Process(es)

structural molecule activity protein binding protein complex binding scaffold protein binding

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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Cytokeratin 8 Polyclonal Antibody

Cytokeratin 8 Antibody (10384-1-AP) in WB

Product Details

10384-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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