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Invitrogen
DAB2 Polyclonal Antibody
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FIGURE: 1 / 12
DAB2 Antibody (PA5-56005) in ICC/IF
Immunofluorescence analysis of DAB2 was performed using 70% confluent log phase RAW 264.7cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DAB2 Polyclonal Antibody (Product # PA5-56005) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjugate (Product #... View More
Product Details
PA5-56005
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human DAB2. Recombinant protein control fragment (Product #RP-102273).
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Immunogen sequence: NAFSANLNFF PTPNPDPFRD DPFTQPDQST PSSFDSLKSP DQKKENSSSS STPLSNGPLN GDVDYFGQQF DQISNRTGKQ EAQAGPWPFS SSQTQPAVRT QNGVSEREQN GFSVKSSPNP
Highest antigen sequence identity to the following orthologs: Mouse - 86%, Rat - 82%.
Target Information
DAB2 mRNA is expressed in normal ovarian epithelial cells but is down-regulated or absent from ovarian carcinoma cell lines. The 770-amino acid predicted protein has an overall 83% identity with the mouse p96 protein, a putative mitogen-responsive phosphoprotein; homology is strongest in the amino-terminal end of the protein in a region corresponding to the phosphotyrosine interaction domain. The down-regulation of DAB2 may play an important role in ovarian carcinogenesis. This gene was initially named DOC2 (for Differentially expressed in Ovarian Cancer) and is distinct from the DOC2A and DOC2B genes (for double C2-like domains, alpha and beta).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Adaptor molecule disabled-2; Dab, mitogen-responsive phosphoprotein, homolog 2; Differentially expressed in ovarian carcinoma 2; Differentially-expressed protein 2; Disabled homolog 2; disabled homolog 2, mitogen-responsive phosphoprotein; DOC-2; FLJ26626
Gene Aliases: DAB2; DOC-2; DOC2
UniProt ID: (Human) P98082
Entrez Gene ID: (Human) 1601
positive regulation of protein phosphorylation
receptor-mediated endocytosis
apoptotic process
integrin-mediated signaling pathway
multicellular organism development
cell proliferation
positive regulation of epithelial to mesenchymal transition
positive regulation of pathway-restricted SMAD protein phosphorylation
protein transport
Wnt signaling pathway
positive regulation of cell migration
positive regulation of transforming growth factor beta receptor signaling pathway
negative regulation of protein binding
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
leading edge cell differentiation
negative regulation of apoptotic process
positive regulation of endocytosis
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of SMAD protein import into nucleus
negative regulation of androgen receptor signaling pathway
negative regulation of canonical Wnt signaling pathway
negative regulation of protein localization to plasma membrane
positive regulation of Wnt signaling pathway, planar cell polarity pathway
positive regulation of clathrin-mediated endocytosis
positive regulation of early endosome to late endosome transport
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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