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Agrisera
DCL2/Dicer-like protein 2 Polyclonal Antibody
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FIGURE: 1 / 1
DCL2/Dicer-like protein 2 Antibody (AS15-3100) in WB
50 µg of total protein from Arabidopsis thaliana whole vegetative rosette, DCL 2 overexpression line (a), wild type Col-0 (b), dcl 2-1 intron insertion (c), extracted with extraction buffer (50 m M Tris p H 7.5; 150 m M Na Cl; 1 m M EDTA; 10% v/v Glycerin; 1 m M DTT, 1 x Complete Protease Inhibitor Cocktail, Roche) and denatured with LaemmLi buffer at 95°C/5 min. were separated on 7.5% SDS-PAGE and blotted ... View More
Product Details
AS15-3100
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from Arabidopsis thaliana DCL2 sequence.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
DCL2 encodes a predominantly nuclear, Dicer-like RNase III endoribonuclease that functions in RNA interference pathways by recognizing double-stranded RNA substrates and processing them into small interfering RNAs, with a prominent role in antiviral silencing that becomes especially evident when the related DCL4 pathway is compromised; it can also contribute to endogenous small-RNA production, including trans-acting siRNA biogenesis, and has been implicated in maintaining aspects of DNA methylation-linked silencing. The DCL2 protein is a large, multidomain enzyme with an N-terminal DExD/H-box helicase module (ATP-dependent RNA remodeling), followed by a DUF283 region, a PAZ domain that helps engage RNA termini, two tandem RNase III catalytic domains that form the dimeric active center for staggered dsRNA cleavage, and C-terminal double-stranded RNA-binding domain(s) that stabilize substrate interactions; multiple alternatively spliced transcript variants are reported, consistent with potential isoform-level modulation of activity and/or regulation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT3G03300; ATDCL2; DICER-LIKE 2; T17B22.28
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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