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Product Details
11380-1-AP
| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:500-1:2400 | View 2 publications 2 publications |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:20-1:200 | - |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
DDB1 Fusion Protein Ag1901 (1-400 aa encoded by BC011686) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.13 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.02% sodium azide |
Storage conditions |
-20°C |
Product Specific Information
This antibody is specific to DDB1.
Immunogen sequence: MSYNYVVTA QKPTAVNGCV TGHFTSAEDL NLLIAKNTRL EIYVVTAEGL RPVKEVGMYG KIAVMELFRP KGESKDLLFI LTAKYNACIL EYKQSGESID IITRAHGNVQ DRIGRPSETG IIGIIDPECR MIGLRLYDGL FKVIPLDRDN KELKAFNIRL EELHVIDVKF LYGCQAPTIC FVYQDPQGRH VKTYEVSLRE KEFNKGPWKQ ENVEAEASMV IAVPEPFGGA IIIGQESITY HNGDKYLAIA PPIIKQSTIV CHNRVDPNGS RYLLGDMEGR LFMLLLEKEE QMDGTVTLKD LRVELLGETS IAECLTYLDN GVVFVGSRLG DSQLVKLNVD SNEQGSYVVA METFTNLGPI VDMCVVDLER QGQGQLVTCS GAFKEGSLRI IRNGIGIHEH A (1-400 aa encoded by BC011686)
Target Information
The DNA damage-binding protein from HeLa cells is associated with polypeptides of relative mass 124,000 (DDB1) and 41,000 (DDB2) as determined by SDS-polyacrylamide gels. To test whether the DNA-repair defect in the subset of XPE patients that lack DNA damage-binding activity is caused by a defect in DDB, Keeney et al. (1994) injected purified human DDB protein into XPE cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: damage specific DNA binding protein 1; damage-specific DNA binding protein 1, 127kDa; Damage-specific DNA-binding protein 1; damage-specific DNA-binding protein, DNA repair; damaged-DNA recognition protein 1; DDB p127 subunit; DDBa; DNA damage-binding protein 1; DNA damage-binding protein a; DNA repair protein; HBV X-associated protein 1; UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1; UV-DDB 1; XAP-1; xeroderma pigmento; Xeroderma pigmentosum group E-complementing protein; XPCe; XPE-BF; XPE-binding factor
Gene Aliases: 127kDa; AA408517; DDB1; DDBA; p127-Ddb1; UV-DDB1; XAP1; XPCE; XPE; XPE-BF
UniProt ID: (Human) Q16531, (Mouse) Q3U1J4
Entrez Gene ID: (Human) 1642, (Mouse) 13194, (Rat) 64470
Molecular Function:
RNA processing factor
nucleotide-excision repair, DNA damage recognition
nucleotide-excision repair, DNA duplex unwinding
DNA repair
transcription-coupled nucleotide-excision repair
nucleotide-excision repair
nucleotide-excision repair, preincision complex stabilization
nucleotide-excision repair, preincision complex assembly
nucleotide-excision repair, DNA incision, 3'-to lesion
nucleotide-excision repair, DNA incision, 5'-to lesion
viral process
Wnt signaling pathway
nucleotide-excision repair, DNA incision
histone H2A monoubiquitination
DNA damage response, detection of DNA damage
protein ubiquitination involved in ubiquitin-dependent protein catabolic process
negative regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
positive regulation of viral genome replication
positive regulation by virus of viral protein levels in host cell
interaction with symbiont
global genome nucleotide-excision repair
UV-damage excision repair
regulation of mitotic cell cycle phase transition
positive regulation of viral release from host cell
cellular response to DNA damage stimulus
protein ubiquitination
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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