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Invitrogen
DDX1 Polyclonal Antibody
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FIGURE: 1 / 3
DDX1 Antibody (PA5-28210) in IHC (P)
DDX1 Polyclonal Antibody detects DDX1 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. DDX1 Polyclonal Antibody (Product # PA5-28210) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Product Details
PA5-28210
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein encompassing a sequence within the center region of human DDX1. The exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
0.76 mg/mL
Amount
76 µg
Purification
Antigen affinity chromatography
Storage buffer
0.1M tris glycine, pH 7, with 10% glycerol
Contains
0.01% thimerosal
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Recommended positive controls: A431, H1299, HeLa, HepG2.
Predicted reactivity: Mouse (97%), Rat (98%), Zebrafish (89%), Xenopus laevis (88%), Chicken (93%), Rhesus Monkey (98%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
DDX1 (ATP-dependent RNA helicase DDX1) acts as an ATP-dependent RNA helicase. It is able to unwind both RNA-RNA and RNA-DNA duplexes. DDX1 possesses 5' single-stranded RNA overhand nuclease activity and ATPase activity on various RNA, but not DNA polynucleotides. DDX1, together with RELA, acts as a coactivator to enhance NF-kappa-b-mediated transcriptional activation. It also acts as a positive transcriptional regulator of cyclin CCND2 expression. It binds to the cyclin CCND2 promoter region. DDX1 is a component of a multi-helicase-TICAM1 complex that acts as a cytoplasmic sensor of viral double stranded RNA and plays a role in activation of a cascade of antiviral responses including the induction of proinflammatory cytokines via the adapter molecule TICAM1.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Asp-Glu-Ala-Asp box; ATP-dependent RNA helicase DDX1; DBP-RB; DEAD (Asp-Glu-Ala-Asp) box helicase 1; DEAD (Asp-Glu-Ala-Asp) box polypeptide 1; DEAD box polypeptide 1; DEAD box protein 1; DEAD box protein retinoblastoma; DEAD box-1; DEAD-box RNA helicase DDX1; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 1; DEAD/H-box helicase 1; unnamed protein product
Gene Aliases: AA409185; DBP-RB; DDX1; UKVH5d
UniProt ID: (Human) Q92499, (Rat) Q641Y8, (Mouse) Q91VR5
Entrez Gene ID: (Human) 1653, (Rat) 84474, (Mouse) 104721
nucleotide binding
nucleic acid binding
DNA binding
chromatin binding
transcription cofactor activity
RNA binding
RNA helicase activity
double-stranded RNA binding
helicase activity
nuclease activity
exonuclease activity
protein binding
ATP binding
poly(A) binding
hydrolase activity
ATPase activity
DNA/RNA helicase activity
ATP-dependent helicase activity
poly(A) RNA binding
ATP-dependent RNA helicase activity
spliceosomal complex assembly
immune system process
positive regulation of myeloid dendritic cell cytokine production
double-strand break repair
tRNA splicing, via endonucleolytic cleavage and ligation
mRNA processing
regulation of translational initiation
tRNA processing
response to virus
positive regulation of I-kappaB kinase/NF-kappaB signaling
response to exogenous dsRNA
innate immune response
defense response to virus
protein localization to cytoplasmic stress granule
transcription, DNA-templated
regulation of transcription, DNA-templated
multicellular organism development
DNA duplex unwinding
nucleic acid phosphodiester bond hydrolysis
RNA secondary structure unwinding
regulation of nucleic acid-templated transcription
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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