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| Applications | Tested Dilution | Publications |
|---|---|---|
Western Blot (WB) |
1:1,000-1:4,000 | View 9 publications 9 publications |
Immunohistochemistry (IHC) |
- | View 1 publication 1 publication |
Immunohistochemistry (Paraffin) (IHC (P)) |
1:20-1:200 | - |
Immunocytochemistry (ICC/IF) |
1:20-1:200 | View 2 publications 2 publications |
Immunoprecipitation (IP) |
0.5-4.0 µg | - |
ChIP assay (ChIP) |
Assay-dependent | View 1 publication 1 publication |
| Product Specifications | |
|---|---|
Species Reactivity |
Human, Mouse, Rat |
Published species |
Human |
Host/Isotype |
Rabbit / IgG |
Class |
Polyclonal |
Type |
Antibody |
Immunogen |
DDX21 Fusion Protein Ag0804 (1-180 aa encoded by BC008071) |
Conjugate |
Unconjugated |
Form |
Liquid |
Concentration |
0.43 mg/mL |
Purification |
Antigen affinity chromatography |
Storage buffer |
PBS, pH 7.3, with 50% glycerol |
Contains |
0.1% sodium azide |
Storage conditions |
-20°C |
DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa. 10528-1-AP is a rabbit polyclonal antibody raised against the N-terminal of human DDX21.
Immunogen sequence: MPGKLRSDA GLESDTAMKK GETLRKQTEE KEKKEKPKSD KTEEIAEEEE TVFPKAKQVK KKAEPSEVDM NSPKSKKAKK KEEPSQNDIS PKTKSLRKKK EPIEKKVVSS KTKKVTKNEE PSEEEIDAPK PKKMKKEKEM NGETREKSPK LKNGFPHPEP DCNPSEAASE ESNSEIEQEI P (1-180 aa encoded by BC008071)
Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: DEAD (Asp-Glu-Ala-Asp) box helicase 21; DEAD (Asp-Glu-Ala-Asp) box polypeptide 21; DEAD (Asp-Glu-Ala-Asp) box polypeptide 21a; DEAD box protein 21; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21 (RNA helicase II/Gu); Gu protein; Gu-alpha; Nucleolar RNA helicase 2; Nucleolar RNA helicase Gu; Nucleolar RNA helicase II; RH II/Gu; RH-II/Gualpha; RNA helicase II/Gu alpha
Gene Aliases: AI255159; AL022742; D10Ertd645e; D10Wsu42e; DDX21; Ddx21a; GUA; GURDB; RH-II/GU; RH-II/GuA
UniProt ID: (Human) Q9NR30, (Rat) Q3B8Q1, (Mouse) Q9JIK5
Entrez Gene ID: (Human) 9188, (Rat) 317399, (Mouse) 56200
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