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          • Primary Antibodies ›
          • DDX21 Antibodies

          Proteintech

          DDX21 Polyclonal Antibody

          33 References
          View all (20) DDX21 antibodies

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          Datasheet
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          Cite DDX21 Polyclonal Antibody

          • Antibody Testing Data (18)
          DDX21 Antibody in Immunocytochemistry (ICC/IF)
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          DDX21 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 18

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          DDX21 Antibody (10528-1-AP) in ICC/IF

          Immunofluorescent analysis of HepG2 cells, using DDX21 antibody 10528-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          DDX21 Antibody in Immunocytochemistry (ICC/IF)
          DDX21 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DDX21 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          DDX21 Antibody in Western Blot (WB)
          DDX21 Antibody in Western Blot (WB)
          DDX21 Antibody in Western Blot (WB)
          DDX21 Antibody in Western Blot (WB)
          DDX21 Antibody in Western Blot (WB)
          DDX21 Antibody in Western Blot (WB)
          DDX21 Antibody in Western Blot (WB)
          DDX21 Antibody in Western Blot (WB)
          DDX21 Antibody in Western Blot (WB)
          DDX21 Antibody in Western Blot (WB)
          DDX21 Antibody in Western Blot (WB)
          DDX21 Antibody in Western Blot (WB)
          DDX21 Antibody in Western Blot (WB)
          DDX21 Antibody in Western Blot (WB)
          DDX21 Antibody in Immunoprecipitation (IP)

          Product Details

          10528-1-AP

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:1,000-1:4,000
          View 18 publications 18 publications

          Immunohistochemistry (IHC)

          -
          View 3 publications 3 publications

          Immunohistochemistry (Paraffin) (IHC (P))

          1:20-1:200
          -

          Immunocytochemistry (ICC/IF)

          1:20-1:200
          View 7 publications 7 publications

          Immunoprecipitation (IP)

          0.5-4.0 µg
          View 3 publications 3 publications

          ChIP assay (ChIP)

          Assay-dependent
          View 1 publication 1 publication

          Not applicable (N/A)

          -
          View 1 publication 1 publication
          Product Specifications

          Species Reactivity

          Human, Mouse, Rat

          Published species

          Human

          Host/Isotype

          Rabbit / IgG

          Class

          Polyclonal

          Type

          Antibody

          Immunogen

          DDX21 Fusion Protein Ag0804 (1-180 aa encoded by BC008071)
          View immunogen

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          0.43 mg/mL

          Purification

          Antigen affinity chromatography

          Storage buffer

          PBS, pH 7.3, with 50% glycerol

          Contains

          0.02% sodium azide

          Storage conditions

          -20°C

          Shipping conditions

          Wet ice (domestic); Dry ice (international)

          Product Specific Information

          DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa. 10528-1-AP is a rabbit polyclonal antibody raised against the N-terminal of human DDX21.

          Immunogen sequence: MPGKLRSDA GLESDTAMKK GETLRKQTEE KEKKEKPKSD KTEEIAEEEE TVFPKAKQVK KKAEPSEVDM NSPKSKKAKK KEEPSQNDIS PKTKSLRKKK EPIEKKVVSS KTKKVTKNEE PSEEEIDAPK PKKMKKEKEM NGETREKSPK LKNGFPHPEP DCNPSEAASE ESNSEIEQEI P (1-180 aa encoded by BC008071)

          Target Information

          Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: DEAD (Asp-Glu-Ala-Asp) box helicase 21; DEAD (Asp-Glu-Ala-Asp) box polypeptide 21; DEAD (Asp-Glu-Ala-Asp) box polypeptide 21a; DEAD box protein 21; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21 (RNA helicase II/Gu); Gu protein; Gu-alpha; Nucleolar RNA helicase 2; Nucleolar RNA helicase Gu; Nucleolar RNA helicase II; RH II/Gu; RH-II/Gualpha; RNA helicase II/Gu alpha

          View more View less

          Gene Aliases: AI255159; AL022742; D10Ertd645e; D10Wsu42e; DDX21; Ddx21a; GUA; GURDB; RH-II/GU; RH-II/GuA

          View more View less

          UniProt ID: (Human) Q9NR30, (Rat) Q3B8Q1, (Mouse) Q9JIK5

          View more View less

          Entrez Gene ID: (Human) 9188, (Rat) 317399, (Mouse) 56200

          View more View less

          Function(s)
          double-stranded RNA binding ATP-dependent RNA helicase activity protein binding ATP binding rRNA binding snoRNA binding poly(A) RNA binding 7SK snRNA binding RNA binding helicase activity nucleotide binding nucleic acid binding RNA helicase activity hydrolase activity
          Process(es)
          osteoblast differentiation metabolic process response to virus response to exogenous dsRNA rRNA processing transcription from RNA polymerase II promoter RNA secondary structure unwinding positive regulation of gene expression, epigenetic transcription, DNA-templated
          It has to be done as per old AB suggested Products section.
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