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DDX21 Polyclonal Antibody

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DDX21 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of HepG2 cells, using DDX21 antibody 10528-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

DDX21 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 10528-1-AP (DDX21 antibody) at dilution of 1:100 (under 10x lens).

DDX21 Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 10528-1-AP (DDX21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DDX21 Antibody in Immunoprecipitation (IP) — IP result of anti-DDX21 (IP:10528-1-AP, 4ug; Detection:10528-1-AP 1:1000) with COLO 320 cells lysate 2800ug.

Product Details

10528-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:1,000-1:4,000	View 9 publications 9 publications
Immunohistochemistry (Paraffin) (IHC (P))	1:20-1:200	-
Immunocytochemistry (ICC/IF)	1:20-1:200	View 2 publications 2 publications
Immunoprecipitation (IP)	0.5-4.0 µg	-
ChIP assay (ChIP)	Assay-dependent	View 1 publication 1 publication
Immunohistochemistry (IHC)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human, Mouse, Rat
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DDX21 Fusion Protein Ag0804 (1-180 aa encoded by BC008071) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.43 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa. 10528-1-AP is a rabbit polyclonal antibody raised against the N-terminal of human DDX21.

Immunogen sequence: MPGKLRSDA GLESDTAMKK GETLRKQTEE KEKKEKPKSD KTEEIAEEEE TVFPKAKQVK KKAEPSEVDM NSPKSKKAKK KEEPSQNDIS PKTKSLRKKK EPIEKKVVSS KTKKVTKNEE PSEEEIDAPK PKKMKKEKEM NGETREKSPK LKNGFPHPEP DCNPSEAASE ESNSEIEQEI P (1-180 aa encoded by BC008071)

Target Information

Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Survey of DDX21 Activity During Rev/RRE Complex Formation.

Journal of molecular biology

Hammond JA,Zhou L,Lamichhane R,Chu HY,Millar DP,Gerace L,Williamson JR

Fri Feb 16 00:00:00 EST 2018

SLERT Regulates DDX21 Rings Associated with Pol I Transcription.

Cell

Xing YH,Yao RW,Zhang Y,Guo CJ,Jiang S,Xu G,Dong R,Yang L,Chen LL

Thu May 04 00:00:00 EDT 2017

SLERT Regulates DDX21 Rings Associated with Pol I Transcription.

Cell

Xing YH,Yao RW,Zhang Y,Guo CJ,Jiang S,Xu G,Dong R,Yang L,Chen LL

Thu May 04 00:00:00 EDT 2017

SLERT Regulates DDX21 Rings Associated with Pol I Transcription.

Cell

Xing YH,Yao RW,Zhang Y,Guo CJ,Jiang S,Xu G,Dong R,Yang L,Chen LL

Thu May 04 00:00:00 EDT 2017

DDX21 translocates from nucleus to cytoplasm and stimulates the innate immune response due to dengue virus infection.

Biochemical and biophysical research communications

Dong Y,Ye W,Yang J,Han P,Wang Y,Ye C,Weng D,Zhang F,Xu Z,Lei Y

Fri Apr 29 00:00:00 EDT 2016

Identification of C/EBPβ target genes in ALK+ anaplastic large cell lymphoma (ALCL) by gene expression profiling and chromatin immunoprecipitation.

PloS one

Bonzheim I,Irmler M,Klier-Richter M,Steinhilber J,Anastasov N,Schäfer S,Adam P,Beckers J,Raffeld M,Fend F,Quintanilla-Martinez L

Tue Feb 18 00:00:00 EST 2014

Identification of C/EBPβ target genes in ALK+ anaplastic large cell lymphoma (ALCL) by gene expression profiling and chromatin immunoprecipitation.

PloS one

Bonzheim I,Irmler M,Klier-Richter M,Steinhilber J,Anastasov N,Schäfer S,Adam P,Beckers J,Raffeld M,Fend F,Quintanilla-Martinez L

Tue Feb 18 00:00:00 EST 2014

Nucleolar scaffold protein, WDR46, determines the granular compartmental localization of nucleolin and DDX21.

Genes to cells : devoted to molecular & cellular mechanisms

Hirai Y,Louvet E,Oda T,Kumeta M,Watanabe Y,Horigome T,Takeyasu K

Sun Sep 01 00:00:00 EDT 2013

Nucleolar scaffold protein, WDR46, determines the granular compartmental localization of nucleolin and DDX21.

Genes to cells : devoted to molecular & cellular mechanisms

Hirai Y,Louvet E,Oda T,Kumeta M,Watanabe Y,Horigome T,Takeyasu K

Sun Sep 01 00:00:00 EDT 2013

A quantitative proteomic analysis uncovers the relevance of CUL3 in bladder cancer aggressiveness.

PloS one

Grau L,Luque-Garcia JL,González-Peramato P,Theodorescu D,Palou J,Fernandez-Gomez JM,Sánchez-Carbayo M

Tue Jul 02 00:00:00 EDT 2013

Differential effects of AKT1(p.E17K) expression on human mammary luminal epithelial and myoepithelial cells.

Human mutation

Salhia B,Van Cott C,Tegeler T,Polpitiya A,Duquette RA,Gale M,Hostteter G,Petritis K,Carpten J

Wed Aug 01 00:00:00 EDT 2012

Myb-binding protein 1a (Mybbp1a) regulates levels and processing of pre-ribosomal RNA.

The Journal of biological chemistry

Hochstatter J,Hölzel M,Rohrmoser M,Schermelleh L,Leonhardt H,Keough R,Gonda TJ,Imhof A,Eick D,Längst G,Németh A

Fri Jul 13 00:00:00 EDT 2012

Differential protein expression profiling by iTRAQ-two-dimensional LC-MS/MS of human bladder cancer EJ138 cells transfected with the metastasis suppressor KiSS-1 gene.

Molecular & cellular proteomics : MCP

Ruppen I,Grau L,Orenes-Piñero E,Ashman K,Gil M,Algaba F,Bellmunt J,Sánchez-Carbayo M

Fri Oct 01 00:00:00 EDT 2010

Bioinformatics

Protein Aliases: DEAD (Asp-Glu-Ala-Asp) box helicase 21; DEAD (Asp-Glu-Ala-Asp) box polypeptide 21; DEAD (Asp-Glu-Ala-Asp) box polypeptide 21a; DEAD box protein 21; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21 (RNA helicase II/Gu); Gu protein; Gu-alpha; Nucleolar RNA helicase 2; Nucleolar RNA helicase Gu; Nucleolar RNA helicase II; RH II/Gu; RH-II/Gualpha; RNA helicase II/Gu alpha

Gene Aliases: AI255159; AL022742; D10Ertd645e; D10Wsu42e; DDX21; Ddx21a; GUA; GURDB; RH-II/GU; RH-II/GuA

UniProt ID: (Human) Q9NR30, (Rat) Q3B8Q1, (Mouse) Q9JIK5

Entrez Gene ID: (Human) 9188, (Rat) 317399, (Mouse) 56200

Function(s)

osteoblast differentiation metabolic process response to virus response to exogenous dsRNA rRNA processing transcription from RNA polymerase II promoter RNA secondary structure unwinding positive regulation of gene expression, epigenetic transcription, DNA-templated

Process(es)

double-stranded RNA binding ATP-dependent RNA helicase activity protein binding ATP binding rRNA binding snoRNA binding poly(A) RNA binding 7SK snRNA binding RNA binding helicase activity nucleotide binding nucleic acid binding RNA helicase activity hydrolase activity

DDX21 Polyclonal Antibody

Product Details

10528-1-AP

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

ChIP assay (ChIP)

Immunohistochemistry (IHC)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

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