Proteintech

DDX21 Polyclonal Antibody

Product Image Gallery

DDX21 Antibody in Immunofluorescence (IF) — Immunofluorescent analysis of HepG2 cells, using DDX21 antibody 10528-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

DDX21 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry of paraffin-embedded human colon cancer using 10528-1-AP (DDX21 antibody) at dilution of 1:100 (under 10x lens).

DDX21 Antibody in Western Blot (WB) — Mouse testis tissue were subjected to SDS PAGE followed by western blot with 10528-1-AP (DDX21 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DDX21 Antibody in Immunoprecipitation (IP) — IP result of anti-DDX21 (IP:10528-1-AP, 4ug; Detection:10528-1-AP 1:1000) with COLO 320 cells lysate 2800ug.

Product Details

Applications

Tested Dilution

Publications

ChIP assay (ChIP)

Assay-dependent

View 1 publication 1 publication

Immunocytochemistry (ICC)

1:20-1:200

Immunofluorescence (IF)

1:20-1:200

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunoprecipitation (IP)

0.5-4.0 µg

Western Blot (WB)

1:1000-1:4000

View 9 publications 9 publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host / Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DDX21 Fusion Protein Ag0804

Conjugate

Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.1% sodium azide

Storage conditions

-20°C

Product Specific Information

DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa. 10528-1-AP is a rabbit polyclonal antibody raised against the N-terminal of human DDX21.

Target Information

Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: DEAD (Asp-Glu-Ala-Asp) box helicase 21; DEAD (Asp-Glu-Ala-Asp) box polypeptide 21; DEAD (Asp-Glu-Ala-Asp) box polypeptide 21a; DEAD box protein 21; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21 (RNA helicase II/Gu); Gu protein; Gu-alpha; Nucleolar RNA helicase 2; Nucleolar RNA helicase Gu; Nucleolar RNA helicase II; RH II/Gu; RH-II/Gualpha; RNA helicase II/Gu alpha

Gene Aliases: AI255159; AL022742; D10Ertd645e; D10Wsu42e; DDX21; Ddx21a; GUA; GURDB; RH-II/GU; RH-II/GuA

UniProt ID: (Human) Q9NR30, (Rat) Q3B8Q1, (Mouse) Q9JIK5

Entrez Gene ID: (Human) 9188, (Rat) 317399, (Mouse) 56200

Molecular Function: RNA binding protein RNA helicase helicase nucleic acid binding