Proteintech

DDX21 Polyclonal Antibody

DDX21 Antibody in Immunocytochemistry (ICC/IF)

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DDX21 Antibody (10528-1-AP) in ICC/IF

Immunofluorescent analysis of HepG2 cells, using DDX21 antibody 10528-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IGG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

DDX21 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

DDX21 Antibody in Immunoprecipitation (IP)

Product Image Gallery

Product Details

10528-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:4,000

View 18 publications 18 publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200

Immunocytochemistry (ICC/IF)

1:20-1:200

View 7 publications 7 publications

Immunoprecipitation (IP)

0.5-4.0 µg

View 3 publications 3 publications

ChIP assay (ChIP)

Assay-dependent

View 1 publication 1 publication

Not applicable (N/A)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DDX21 Fusion Protein Ag0804 (1-180 aa encoded by BC008071)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.43 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

DDX21 exists as two isoforms, molecular weight of modified isoform one is about 87 -100 kDa, and the post-modified isoform is about 75-85 kDa. 10528-1-AP is a rabbit polyclonal antibody raised against the N-terminal of human DDX21.

Immunogen sequence: MPGKLRSDA GLESDTAMKK GETLRKQTEE KEKKEKPKSD KTEEIAEEEE TVFPKAKQVK KKAEPSEVDM NSPKSKKAKK KEEPSQNDIS PKTKSLRKKK EPIEKKVVSS KTKKVTKNEE PSEEEIDAPK PKKMKKEKEM NGETREKSPK LKNGFPHPEP DCNPSEAASE ESNSEIEQEI P (1-180 aa encoded by BC008071)

Target Information

Eukaryotic initiation factor 2B (EIF2B), which is necessary for protein synthesis, is a GTP exchange factor composed of five different subunits. The protein encoded by this gene is the fourth, or delta, subunit. Defects in this gene are a cause of leukoencephalopathy with vanishing white matter (VWM) and ovarioleukodystrophy. Multiple transcript variants encoding different isoforms have been found for this gene.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges.

iScience

Miyagi T,Ueda K,Sugimoto M,Yagi T,Ito D,Yamazaki R,Narumi S,Hayamizu Y,Uji-I H,Kuroda M,Kanekura K

Fri Jun 16 00:00:00 UTC 2023

Glucose dissociates DDX21 dimers to regulate mRNA splicing and tissue differentiation.

Cell

Miao W,Porter DF,Lopez-Pajares V,Siprashvili Z,Meyers RM,Bai Y,Nguyen DT,Ko LA,Zarnegar BJ,Ferguson ID,Mills MM,Jilly-Rehak CE,Wu CG,Yang YY,Meyers JM,Hong AW,Reynolds DL,Ramanathan M,Tao S,Jiang S,Flynn RA,Wang Y,Nolan GP,Khavari PA

Thu Jan 05 00:00:00 UTC 2023

Glucose dissociates DDX21 dimers to regulate mRNA splicing and tissue differentiation.

Cell

Thu Jan 05 00:00:00 UTC 2023

Ribosome changes reprogram translation for chemosurvival in G0 leukemic cells.

Science advances

Datta C,Truesdell SS,Wu KQ,Bukhari SIA,Ngue H,Buchanan B,Le Tonqueze O,Lee S,Kollu S,Granovetter MA,Boukhali M,Kreuzer J,Batool MS,Balaj L,Haas W,Vasudevan S

Fri Oct 28 00:00:00 UTC 2022

2B and 3C Proteins of Senecavirus A Antagonize the Antiviral Activity of DDX21 via the Caspase-Dependent Degradation of DDX21.

Frontiers in immunology

Zhao K,Guo XR,Liu SF,Liu XN,Han Y,Wang LL,Lei BS,Zhang WC,Li LM,Yuan WZ

Tue Aug 02 00:00:00 UTC 2022

The Roles of RNA Helicases in DNA Damage Repair and Tumorigenesis Reveal Precision Therapeutic Strategies.

Cancer research

Xie J,Wen M,Zhang J,Wang Z,Wang M,Qiu Y,Zhao W,Zhu F,Yao M,Rong Z,Hu W,Pei Q,Sun X,Li J,Mao Z,Sun LQ,Tan R

Tue Mar 01 00:00:00 UTC 2022

The Roles of RNA Helicases in DNA Damage Repair and Tumorigenesis Reveal Precision Therapeutic Strategies.

Cancer research

Xie J,Wen M,Zhang J,Wang Z,Wang M,Qiu Y,Zhao W,Zhu F,Yao M,Rong Z,Hu W,Pei Q,Sun X,Li J,Mao Z,Sun LQ,Tan R

Tue Mar 01 00:00:00 UTC 2022

An eIF3d-dependent switch regulates HCMV replication by remodeling the infected cell translation landscape to mimic chronic ER stress.

Cell reports

Thompson L,Depledge DP,Burgess HM,Mohr I

Tue May 03 00:00:00 UTC 2022

Nuclear microRNAs release paused Pol II via the DDX21-CDK9 complex.

Cell reports

Ohno SI,Oikawa K,Tsurui T,Harada Y,Ono K,Tateishi M,Mirza A,Takanashi M,Kanekura K,Nagase K,Shimada Y,Kudo Y,Ikeda N,Ochiya T,Wang X,Kuroda M

Tue Apr 12 00:00:00 UTC 2022

Nuclear microRNAs release paused Pol II via the DDX21-CDK9 complex.

Cell reports

Ohno SI,Oikawa K,Tsurui T,Harada Y,Ono K,Tateishi M,Mirza A,Takanashi M,Kanekura K,Nagase K,Shimada Y,Kudo Y,Ikeda N,Ochiya T,Wang X,Kuroda M

Tue Apr 12 00:00:00 UTC 2022

Pan-cancer analysis reveals DDX21 as a potential biomarker for the prognosis of multiple tumor types.

Frontiers in oncology

Hu A,Wang Y,Tian J,Chen Z,Chen R,Han X,Chen Y,Liu T,Chen Q

Thu Dec 22 00:00:00 UTC 2022

Pan-cancer analysis reveals DDX21 as a potential biomarker for the prognosis of multiple tumor types.

Frontiers in oncology

Hu A,Wang Y,Tian J,Chen Z,Chen R,Han X,Chen Y,Liu T,Chen Q

Thu Dec 22 00:00:00 UTC 2022

Pan-cancer analysis reveals DDX21 as a potential biomarker for the prognosis of multiple tumor types.

Frontiers in oncology

Hu A,Wang Y,Tian J,Chen Z,Chen R,Han X,Chen Y,Liu T,Chen Q

Thu Dec 22 00:00:00 UTC 2022

lncRNA SLERT controls phase separation of FC/DFCs to facilitate Pol I transcription.

Science (New York, N.Y.)

Wu M,Xu G,Han C,Luan PF,Xing YH,Nan F,Yang LZ,Huang Y,Yang ZH,Shan L,Yang L,Liu J,Chen LL

Fri Jul 30 00:00:00 UTC 2021

Nuclear bodies formed by polyQ-ataxin-1 protein are liquid RNA/protein droplets with tunable dynamics.

Scientific reports

Zhang S,Hinde E,Parkyn Schneider M,Jans DA,Bogoyevitch MA

Fri Jan 31 00:00:00 UTC 2020

PRL3-DDX21 Transcriptional Control of Endolysosomal Genes Restricts Melanocyte Stem Cell Differentiation.

Developmental cell

Johansson JA,Marie KL,Lu Y,Brombin A,Santoriello C,Zeng Z,Zich J,Gautier P,von Kriegsheim A,Brunsdon H,Wheeler AP,Dreger M,Houston DR,Dooley CM,Sims AH,Busch-Nentwich EM,Zon LI,Illingworth RS,Patton EE

Mon Aug 10 00:00:00 UTC 2020

Dissecting the Role of DDX21 in Regulating Human Cytomegalovirus Replication.

Journal of virology

Hao H,Han T,Xuan B,Sun Y,Tang S,Yue N,Qian Z

Sun Dec 15 00:00:00 UTC 2019

Activation of PARP-1 by snoRNAs Controls Ribosome Biogenesis and Cell Growth via the RNA Helicase DDX21.

Molecular cell

Kim DS,Camacho CV,Nagari A,Malladi VS,Challa S,Kraus WL

Thu Sep 19 00:00:00 UTC 2019

Activation of PARP-1 by snoRNAs Controls Ribosome Biogenesis and Cell Growth via the RNA Helicase DDX21.

Molecular cell

Kim DS,Camacho CV,Nagari A,Malladi VS,Challa S,Kraus WL

Thu Sep 19 00:00:00 UTC 2019

Activation of PARP-1 by snoRNAs Controls Ribosome Biogenesis and Cell Growth via the RNA Helicase DDX21.

Molecular cell

Kim DS,Camacho CV,Nagari A,Malladi VS,Challa S,Kraus WL

Thu Sep 19 00:00:00 UTC 2019

A Survey of DDX21 Activity During Rev/RRE Complex Formation.

Journal of molecular biology

Hammond JA,Zhou L,Lamichhane R,Chu HY,Millar DP,Gerace L,Williamson JR

Fri Feb 16 00:00:00 UTC 2018

A Survey of DDX21 Activity During Rev/RRE Complex Formation.

Journal of molecular biology

Hammond JA,Zhou L,Lamichhane R,Chu HY,Millar DP,Gerace L,Williamson JR

Fri Feb 16 00:00:00 UTC 2018

SLERT Regulates DDX21 Rings Associated with Pol I Transcription.

Cell