|Tested species reactivity||Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||KLH conjugated synthetic peptide between 39-68 amino acids from the N-terminal region of human DENND1B|
|Purification||Antigen affinity chromatography|
|Contains||0.09% sodium azide|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Flow Cytometry (Flow)||1:10-1:50|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50-1:100|
|Western Blot (WB)||1:1000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with mouse based on sequence homology.
Clathrin (see MIM 118955)-mediated endocytosis is a majormechanism for internalization of proteins and lipids. Members ofthe connecdenn family, such as DENND1B, function as guaninenucleotide exchange factors (GEFs) for the early endosomal smallGTPase RAB35 (MIM 604199) and bind to clathrin and clathrin adaptorprotein-2 (AP2; see MIM 601024). Thus, connecdenns link RAB35activation with the clathrin machinery.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.