Invitrogen

DGCR8 Polyclonal Antibody

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated. View Details

View all (18) DGCR8 antibodies

Datasheet

Questions & Answers

Datasheet

Questions & Answers

Product Image Gallery

DGCR8 Antibody in Western Blot (WB) — Knockdown of DGCR8 was achieved by transfecting HEK-293 cells with DGCR8 specific siRNAs (Silencer® select Product # s29062, s29061). Western blot analysis (Fig. a) was performed using whole cell extracts from the DGCR8 knockdown cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with DGCR8 Antibody (Product # PA5-40122, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 0.25µg/ml, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to DGCR8.

DGCR8 Antibody in Immunoprecipitation (IP) — Immunoprecipitation of DGCR8 in Jurkat whole cell extracts (5µg) using a DGCR8 polyclonal antibody (Product # PA5-40122) followed by Western blot analysis and detection using a Rabbit IgG secondary antibody.

DGCR8 Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of DGCR8 was performed using HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with DGCR8 Polyclonal Antibody (Product # PA5-40122) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 conjugate (Product # A32790) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the composite image showing nuclear localization of DGCR8 in HEK-293 cells. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

DGCR8 Antibody in Knockdown — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK-293 cells were transfected with DGCR8 siRNA and decrease in signal intensity was observed in western blot application using Anti-DGCR8 Polyclonal Antibody (Product # PA5-40122). {KD}

Product Details

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:3000

Immunoprecipitation (IP)

1:100-1:500

Immunocytochemistry (ICC/IF)

1:100

Product Specifications

Species Reactivity

Amphibian, Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human DGCR8.

Conjugate

Unconjugated

Form

Liquid

Concentration

1.15 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_2606318

Product Specific Information

Recommended positive controls: 293T, A431, HeLa, and HepG2 cells.

Predicted reactivity: Mouse (82%), Rat (80%), Rhesus Monkey (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.