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Product Details
PA5-53558
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein corresponding to Human DGKE. Recombinant protein control fragment (Product #RP-90372).
Conjugate
Form
Liquid
Concentration
0.1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.2, with 40% glycerol
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Wet ice
RRID
AB_2640547
Product Specific Information
Immunogen sequence: RDTDLFSQPT YCCVCAQHIL QGAFCDCCGL RVDEGCLRKA DKRFQCKEIM LKNDTKVLDA MPHHWIRGNV PLCSYCMVCK QQCGCQPKLC DYRCIWCQKT VHDECMKNSL KNEKCDFGEF KNLIIPPSYL
Highest antigen sequence identity to the following orthologs: Mouse - 88%, Rat - 86%.
Target Information
Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-alpha (80 kDa), -beta (90 kDa), and - gamma (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK- delta (130 kDa) and DGK-i (130 kDa) contain N-terminal pleckstrin homology (PH) domains and are classified as type II. Human DGK- epsilon (64 kDa) contains no identifiable regulatory domains and is classified as a type III DGK. Human DGK- zeta (104 kDa) and -iota (130 kDa) possess C-terminal ankyrin repeats and are classified as type IV DGKs. Human DGK-theta (110 kDa) contains 3 cysteine-rich domains and a PH domain and is classified as a type V DGK.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DAG kinase epsilon; DGK-epsilon; Diacylglycerol kinase epsilon; diacylglycerol kinase, epsilon 64kDa; Diglyceride kinase epsilon; EC 2.7.1.107
Gene Aliases: AHUS7; DAGK5; DAGK6; DGK; DGKE; NPHS7
UniProt ID: (Human) P52429
Entrez Gene ID: (Human) 8526
Performance Guarantee
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