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FIGURE: 1 / 1
DGKZ Antibody (PA5-120452) in WB
Western blot analysis of DGKZ in extracts of various cell lines. Samples were incubated with DGKZ Polyclonal antibody (Product # PA5-120452) using a dilution of 1:300, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-120452
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 808-1117 of human DGKZ (NP_001099010.1).
Conjugate
Form
Liquid
Concentration
0.22 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2914024
Product Specific Information
Positive test controls include: LO2, HeLa, 293T, Mouse brain. The target is usually found in the following locations: Cell membrane, Cytoplasm, Nucleus.
Immunogen sequence: ATMVQKAKRR SAAPLHSDQQ PVPEQLRIQV SRVSMHDYEA LHYDKEQLKE ASVPLGTVVV PGDSDLELCR AHIERLQQEP DGAGAKSPTC QKLSPKWCFL DATTASRFYR IDRAQEHLNY VTEIAQDEIY ILDPELLGAS ARPDLPTPTS PLPTSPCSPT PRSLQGDAAP PQGEELIEAA KRNDFCKLQE LHRAGGDLMH RDEQSRTLLH HAVSTGSKDV VRYLLDHAPP EILDAVEENG ETCLHQAAAL GQRTICHYIV EAGASLMKTD QQGDTPRQRA EKAQDTELAA YLENRQHYQM IQREDQETAV
Target Information
The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It may attenuate protein kinase C activity by regulating diacylglycerol levels in intracellular signaling cascade and signal transduction. Alternative splicing occurs at this locus and four transcript variants encoding distinct isoforms have been identified.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DAG kinase DAGK6; DAG kinase zeta; DGK-zeta; Diacylglycerol kinase zeta; diacylglycerol kinase, zeta 104kDa; Diglyceride kinase; Diglyceride kinase zeta
Gene Aliases: DAGK5; DAGK6; DGK-ZETA; DGKZ; E130307B02Rik; ENSMUSG00000075035; F730209L11Rik; hDGKzeta; mDGK[z]
UniProt ID: (Human) Q13574, (Mouse) Q80UP3
Entrez Gene ID: (Human) 8525, (Mouse) 104418
protein kinase C-activating G-protein coupled receptor signaling pathway
phosphorylation
cell migration
platelet activation
intracellular signal transduction
mitotic G1 DNA damage checkpoint
negative regulation of catalytic activity
negative regulation of mitotic cell cycle
negative regulation of Ras protein signal transduction
lipid phosphorylation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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