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Agrisera
DHAR1/Dehydroascorbate Reductase 1 Polyclonal Antibody
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FIGURE: 1 / 1
DHAR1/Dehydroascorbate Reductase 1 Antibody (AS11-1746) in WB
1 cm 2 of a leaf from Arabidopsis thaliana Col-0 (1) and or t-DNA insertion lines dhar 1-1 (2), dhar 1-2 (3), dhar 1-3 (4), dhar 2-1 (5), dhar 2-2 (6), dhar 1-3 EOS-DHAR 1 (7), was extracted using 200 µL Lyse& Load-Buffer (Grefen et al. 2009). 10 µL were separated on a 15% SDS-PAGE and blotted 1 h to PVDF (using Bjerrum Buffer in a semidry blot). Blots were blocked with 5% Milk in 1 x TBS-Tween 20 (1%) for ... View More
Product Details
AS11-1746
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated synthetic peptide derived from known DHAR1 sequence of Arabidopsis thaliana.
Conjugate
Form
Lyophilized
Amount
200 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 200 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Kandelia candel, Solanum lycopersicum
Target Information
DHAR1 encodes a dehydroascorbate reductase that functions as a soluble, cytosolic enzyme in Arabidopsis thaliana, contributing to cellular redox homeostasis by supporting the ascorbate-glutathione cycle. The DHAR1 protein is a small (~213 aa) glutathione-dependent oxidoreductase with a thioredoxin-like fold and glutathione S-transferase-like N- and C-terminal regions typical of plant DHARs, consistent with its role in binding glutathione and reducing dehydroascorbate back to ascorbate. Genetically, loss of DHAR1 reduces total DHAR activity in leaves and compromises maintenance of the ascorbate redox state during photooxidative stress, leading to heightened stress symptoms compared with wild type, indicating DHAR1 is a key enzymatic component for ascorbate recycling and protection against reactive oxygen species in planta.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ATDHAR1; dehydroascorbate reductase; DEHYDROASCORBATE REDUCTASE 5; DHAR5; F14P1.9; F14P1_9
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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