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Invitrogen

DHX9 Polyclonal Antibody

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DHX9 Polyclonal Antibody

Product Details

PA5-85679

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:3,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-

Immunocytochemistry (ICC/IF)

1:100-1:1,000
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DHX9. The exact sequence is proprietary.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.35 mg/mL

Amount

35 µg

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol, 1% BSA

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2792818

Product Specific Information

Keep as concentrated solution.

Predicted reactivity: Rhesus Monkey (93%).

Positive Control: HeLa, HeLa nuclear extract.

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

The human alanyl-tRNA synthetase (AARS) belongs to a family of tRNA synthases, of the class II enzymes. Class II tRNA synthases evolved early in evolution and are highly conserved. This is reflected by the fact that 498 of the 968-residue polypeptide human AARS shares 41% identity witht the E.coli protein. tRNA synthases are the enzymes that interpret the RNA code and attach specific aminoacids to the tRNAs that contain the cognate trinucleotide anticodons. They consist of a catalytic domain which interacts with the amino acid acceptor-T psi C helix of the tRNA, and a second domain which interacts with the rest of the tRNA structure.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: ATP-dependent RNA helicase A; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 9; DEAH (Asp-Glu-Ala-His) box helicase 9; DEAH (Asp-Glu-Ala-His) box polypeptide 9; DEAH box protein 9; DEAH-box helicase 9; DExH-box helicase 9; FLJ17406; Leukophysin; LKP; NDH II; Nuclear DNA helicase II; OTTHUMP00000033287; RNA helicase A; unnamed protein product

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Gene Aliases: DDX9; DHX9; LKP; MRD75; NDH2; NDHII; RHA

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UniProt ID: (Human) Q08211

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Entrez Gene ID: (Human) 1660

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Function(s)
nucleotide binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II core binding regulatory region RNA binding nucleic acid binding DNA binding DNA helicase activity DNA replication origin binding double-stranded DNA binding single-stranded DNA binding transcription cofactor activity transcription coactivator activity RNA binding RNA helicase activity double-stranded RNA binding single-stranded RNA binding mRNA binding helicase activity protein binding ATP binding hydrolase activity ATPase activity nucleoside-triphosphatase activity chromatin DNA binding 3'-5' DNA/RNA helicase activity 3'-5' RNA helicase activity siRNA binding RNA stem-loop binding ribosome binding 3'-5' DNA helicase activity triplex DNA binding metal ion binding nucleoside-triphosphate diphosphatase activity RNA polymerase II sequence-specific DNA binding transcription factor binding importin-alpha family protein binding RNA polymerase binding catalytic activity, acting on a nucleic acid RISC complex binding single-stranded DNA-dependent ATP-dependent 3'-5' DNA helicase activity sequence-specific mRNA binding promoter-specific chromatin binding
Process(es)
alternative mRNA splicing, via spliceosome osteoblast differentiation immune system process DNA replication chromatin organization DNA-templated transcription, termination regulation of transcription from RNA polymerase II promoter mRNA processing regulation of translation inflammatory response RNA splicing gene silencing by RNA positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interleukin-18 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production gene silencing by miRNA DNA-templated viral transcription innate immune response positive regulation of innate immune response positive regulation of DNA repair positive regulation of DNA replication positive regulation of transcription from RNA polymerase II promoter positive regulation of RNA export from nucleus positive regulation of fibroblast proliferation rhythmic process positive regulation of viral transcription regulation of mRNA processing regulation of defense response to virus by host positive regulation of inflammatory response mRNA transport positive regulation of NF-kappaB transcription factor activity positive regulation of response to cytokine stimulus protein-containing complex assembly pyroptosis small RNA loading onto RISC CRD-mediated mRNA stabilization cellular response to exogenous dsRNA negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay protein localization to cytoplasmic stress granule regulation of cytoplasmic translation positive regulation of cytoplasmic translation
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