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Invitrogen
DIAPH2 Polyclonal Antibody
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FIGURE: 1 / 1
DIAPH2 Antibody (PA5-120072) in WB
Western blot analysis of DIAPH2 in extracts of various cell lines. Samples were incubated with DIAPH2 Polyclonal antibody (Product # PA5-120072) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.
Product Details
PA5-120072
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human DIAPH2 (NP_009293.1).
Conjugate
Form
Liquid
Concentration
1.41 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive test controls include: SKOV3, SW620, NCI-H460, LO2, 293T, Mouse liver, Mouse kidney, Rat liver. The target is usually found in the following locations: Cytoplasm, Early endosome, cytosol.
Immunogen sequence: MEQPGAAASG AGGGSEEPGG GRSNKRSAGN RAANEEETKN KPKLNIQIKT LADDVRDRIT SFRKSTVKKE KPLIQHPIDS QVAMSEFPAA QPLYDERSLN LSEKEVLDLF EKMMEDMNLN
Target Information
The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: diaphanous homolog 2; diaphanous-related formin 2; Diaphanous-related formin-2; diaphorase-2; DRF2; FLJ11167; mDia3; mDia3 isoform; Protein diaphanous homolog 2; RP11-212B22.1
Gene Aliases: DIA; DIA2; Dia3; Diap2; DIAPH2; DRF2; E430022I22Rik; POF; POF2; RGD1564399
UniProt ID: (Human) O60879, (Mouse) O70566
Entrez Gene ID: (Human) 1730, (Mouse) 54004, (Rat) 317189
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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