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DLL4 Polyclonal Antibody

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DLL4 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA)) — Immunofluorescent analysis of ( 4% PFA) fixed human stomach cancer tissue using 21584-1-AP (DLL4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

DLL4 Antibody in Western Blot (WB) — Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 21584-1-AP ( DLL4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

DLL4 Antibody in Immunoprecipitation (IP) — IP result of anti-DLL4 (IP:21584-1-AP, 4ug; Detection:21584-1-AP 1:300) with mouse lung tissue lysate 4000 ug.

Product Details

21584-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:1,000

View 13 publications 13 publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:50-1:500

View 1 publication 1 publication

Immunoprecipitation (IP)

0.5-4.0 µg

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

DLL4 Fusion Protein Ag16140 (317-530 aa encoded by BC106950)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

0.22 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Product Specific Information

Immunogen sequence: TGVDCELEL SECDSNPCRN GGSCKDQEDG YHCLCPPGYY GLHCEHSTLS CADSPCFNGG SCRERNQGAN YACECPPNFT GSNCEKKVDR CTSNPCANGG QCLNRGPSRM CRCRPGFTGT YCELHVSDCA RNPCAHGGTC HDLENGLMCT CPAGFSGRRC EVRTSIDACA SSPCFNRATC YTDLSTDTFV CNCPYGFVGS RCEFPVGLPP SFPWV (317-530 aa encoded by BC106950)

Target Information

Delta-like ligand 4 (DLL4), also know as Delta-4, is a homologue of the Drosophila delta protein, and functions as a transmembrane bound ligand to the notch receptor Notch1. DLL4 is expressed in the vasculature and plays a critical role in vascular development. It is induced by vascular endothelial growth factor (VEGF), as a negative feedback regulator to regulate angiogenic sprouting and promote the formulation of a differentiated vascular network. DLL4 has been found to be strongly expressed in tumor vessels of clear-cell renal tumors and bladder cancer, and inhibition of DLL4 results in increased vascular proliferation but defective maturation. This in turn leads to a decrease in tumor growth, with no apparent toxicity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

APEX1 predicts poor prognosis of gallbladder cancer and affects biological properties of CD133⁺ GBC-SD cells via upregulating Jagged1.

Journal of Cancer

Wu Z,Liu Z,Sun Y,Yuan Y,Zou Q,Wen Y,Luo J,Liu R

Thu Jun 08 00:00:00 EDT 2023

Endothelial-Specific Molecule 1 Inhibition Lessens Productive Angiogenesis and Tumor Metastasis to Overcome Bevacizumab Resistance.

Cancers

Kang N,Liang X,Fan B,Zhao C,Shen B,Ji X,Liu Y

Fri Nov 18 00:00:00 EST 2022

Endothelial-Specific Molecule 1 Inhibition Lessens Productive Angiogenesis and Tumor Metastasis to Overcome Bevacizumab Resistance.

Cancers

Kang N,Liang X,Fan B,Zhao C,Shen B,Ji X,Liu Y

Fri Nov 18 00:00:00 EST 2022

Endothelial-Specific Molecule 1 Inhibition Lessens Productive Angiogenesis and Tumor Metastasis to Overcome Bevacizumab Resistance.

Cancers

Kang N,Liang X,Fan B,Zhao C,Shen B,Ji X,Liu Y

Fri Nov 18 00:00:00 EST 2022

Endothelial-Specific Molecule 1 Inhibition Lessens Productive Angiogenesis and Tumor Metastasis to Overcome Bevacizumab Resistance.

Cancers

Kang N,Liang X,Fan B,Zhao C,Shen B,Ji X,Liu Y

Fri Nov 18 00:00:00 EST 2022

Atractylenolide III Attenuates Angiogenesis in Gastric Precancerous Lesions Through the Downregulation of Delta-Like Ligand 4.

Frontiers in pharmacology

Gao Y,Wang J,Zhao M,Xia T,Liu Q,Chen N,Liao W,Zeng Z,You F,Zeng J

Tue Jul 19 00:00:00 EDT 2022

Dysregulation of the miR-30c/DLL4 axis by circHIPK3 is essential for KSHV lytic replication.

EMBO reports

Harper KL,Mottram TJ,Anene CA,Foster B,Patterson MR,McDonnell E,Macdonald A,Westhead D,Whitehouse A

Wed May 04 00:00:00 EDT 2022

Endothelial PERK-ATF4-JAG1 axis activated by T-ALL remodels bone marrow vascular niche.

Theranostics

Liu C,Chen Q,Shang Y,Chen L,Myers J,Awadallah A,Sun J,Yu S,Umphred-Wilson K,Che D,Dou Y,Li L,Wearsch P,Ramírez-Bergeron D,Beck R,Xin W,Jin G,Adoro S,Zhou L

Tue Apr 12 00:00:00 EDT 2022

Electrochemically derived nanographene oxide activates endothelial tip cells and promotes angiogenesis by binding endogenous lysophosphatidic acid.

Bioactive materials

Liu W,Luo H,Wei Q,Liu J,Wu J,Zhang Y,Chen L,Ren W,Shao L

Tue Mar 01 00:00:00 EST 2022

Role of the Notch ligands Jagged1 and Delta4 in Th17/Treg immune imbalance in a mouse model of chronic asthma.

Experimental lung research

He Z,Wu J,Zeng X,Bao H,Liu X

Thu Jan 13 00:00:00 EST 2022

Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Xu XY,Choi HS,Park SY,Kim JK,Seo KH,Kim H,Kim YJ

Sat Jan 01 00:00:00 EST 2022

Hibiscus syriacus L. cultivated in callus culture exerts cytotoxicity in colorectal cancer via Notch signaling-mediated cholesterol biosynthesis suppression.

Phytomedicine : international journal of phytotherapy and phytopharmacology

Xu XY,Choi HS,Park SY,Kim JK,Seo KH,Kim H,Kim YJ

Sat Jan 01 00:00:00 EST 2022

Knockdown of growth factor receptor bound protein 7 suppresses angiogenesis by inhibiting the secretion of vascular endothelial growth factor A in ovarian cancer cells.

Bioengineered

Xu Q,Liu Z,Zhu ZQ,Fan Y,Chen R,Xie XH,Cheng M

Wed Dec 01 00:00:00 EST 2021

Anti-DLL4 ameliorates toluene diisocyanate-induced experimental asthma by inhibiting Th17 response.

International immunopharmacology

Deng Y,Chen S,Song S,Huang Y,Chen R,Tao A

Sat May 01 00:00:00 EDT 2021

Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes.

Angiogenesis

Bai J,Khajavi M,Sui L,Fu H,Tarakkad Krishnaji S,Birsner AE,Bazinet L,Kamm RD,D'Amato RJ

Mon Feb 01 00:00:00 EST 2021

Angiogenic responses in a 3D micro-engineered environment of primary endothelial cells and pericytes.

Angiogenesis

Bai J,Khajavi M,Sui L,Fu H,Tarakkad Krishnaji S,Birsner AE,Bazinet L,Kamm RD,D'Amato RJ

Mon Feb 01 00:00:00 EST 2021

Long non-coding RNA GAPLINC promotes angiogenesis by regulating miR-211 under hypoxia in human umbilical vein endothelial cells.

Journal of cellular and molecular medicine

He Y,Wu Z,Qiu C,Wang X,Xiang Y,Lu T,He Y,Shang T,Zhu Q,Wang X,Zeng Q,Zhang H,Li D

Sun Dec 01 00:00:00 EST 2019

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Cell metabolism

Zhong X,Cui P,Cai Y,Wang L,He X,Long P,Lu K,Yan R,Zhang Y,Pan X,Zhao X,Li W,Zhang H,Zhou Q,Gao P

Tue Apr 02 00:00:00 EDT 2019

Mitochondrial Dynamics Is Critical for the Full Pluripotency and Embryonic Developmental Potential of Pluripotent Stem Cells.

Cell metabolism

Zhong X,Cui P,Cai Y,Wang L,He X,Long P,Lu K,Yan R,Zhang Y,Pan X,Zhao X,Li W,Zhang H,Zhou Q,Gao P

Tue Apr 02 00:00:00 EDT 2019

Bioinformatics

Protein Aliases: delta 4; delta ligand 4; delta-like 4 homolog; delta-like 4 protein; Delta-like protein 4; Delta4; Drosophila Delta homolog 4; MGC126344; notch ligand delta-2; notch ligand DLL4

Gene Aliases: AOS6; Delta4; DLL4; hdelta2; UNQ1895/PRO4341

UniProt ID: (Human) Q9NR61, (Mouse) Q9JI71

Entrez Gene ID: (Human) 54567, (Mouse) 54485

Function(s)

Process(es)

Notch binding calcium ion binding protein binding

DLL4 Polyclonal Antibody

Product Details

21584-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Immunoprecipitation (IP)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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