Heat-mediated antigen retrieval using an antigen unmasking fluid is recommended, followed by a 2-hr incubation with primary antibody.
Rabbit anti DNA PKcs antibody recognizes the human DNA-dependent protein kinase catalytic subunit, also known as DNA-PKcs, DNPK1 or p460.
This gene encodes a component of the mediator complex, a transcriptional coactivator complex thought to be required for the expression of almost all genes. The mediator complex is recruited by transcriptional activators or nuclear receptors to induce gene expression, possibly by interacting with RNA polymerase II and promoting the formation of a transcriptional pre-initiation complex. Multiple transcript variants encoding different isoforms have been found for this gene.
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Protein Aliases: DNA-dependent protein kinase catalytic subunit; DNA-PK catalytic subunit; DNPK1; doxorubicin nephropathy; hyper-radiosensitivity of murine scid mutation, complementing 1; p460; protein kinase, DNA activated, catalytic polypeptide; protein kinase, DNA-activated, catalytic polypeptide; severe combined immunodeficiency
Gene Aliases: AI326420; AU019811; DNA-PKcs; DNAPDcs; DNAPK; DNPK1; DOXNPH; dxnph; HYRC; HYRC1; I79_017613; IMD26; p350; p460; PRKDC; scid; slip; XRCC7