Invitrogen

DNAH1 Polyclonal Antibody

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DNAH1 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of DNAH1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115132) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).

DNAH1 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of DNAH1 in rat kidney tissue. Samples were treated with formaldehyde and treated with citrate buffer for antigen retrieval, blocked, and incubated (1.5 hours at 22°C) with polyclonal antibody (Product # PA5-115132) at a dilution of 1:100. Secondary staining was applied with HRP conjugated anti-Rabbit.

DNAH1 Antibody in Western Blot (WB) — Western blot analysis of DNAH1 in human nasopharynx (left lane: treated with blocking peptide). Samples were incubated with polyclonal antibody (Product # PA5-115132).

Product Details

PA5-115132

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	-
Immunohistochemistry (PFA fixed) (IHC (PFA))	1:50-1:200	-
Immunocytochemistry (ICC/IF)	1:100-1:500	-

Product Specifications
Species Reactivity	Human, Rat
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	A synthesized peptide derived from human DNAH1(Accession Q9P2D7), corresponding to amino acid residues G3133-A3162. View antigen
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	Affinity chromatography
Storage buffer	PBS with 150mM NaCl, 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: Axonemal beta dynein heavy chain 1; Ciliary dynein heavy chain 1; DNAH1 variant protein; Dynein heavy chain 1, axonemal; dynein, axonemal, heavy chain 1; dynein, axonemal, heavy polypeptide 1; hDHC7; Heat shock regulated protein 1; HSRF-1; testicular tissue protein Li 60

Gene Aliases: DHC7; Dlp1; DNAH1; DNAHC1; HDHC7; HL-11; HL11; HSRF-1; KIAA1410; RGD1311110; XLHSRF-1

UniProt ID: (Human) Q9P2D7, (Rat) Q63164

Entrez Gene ID: (Human) 25981, (Rat) 171339

Molecular Function: cytoskeletal protein microtubule binding motor protein microtubule or microtubule-binding cytoskeletal protein