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Invitrogen
DNM1L Polyclonal Antibody
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FIGURE: 1 / 4
DNM1L Antibody (PA5-144673) in ICC/IF
Immunocytochemistry analysis of DNM1L in NIH/3T3 cells. Cells were fixed in 4% paraformaldehyde for 10 minutes at 37 &(8)451;, permeabilized with 0.05% Triton X-100 in PBS for 20 minutes, and then blocked with 2% negative goat serum for 30 minutes at room temperature. Cells were incubated with DNM1L polyclonal antibody (Product # PA5-144673) with a dilution of 1:200 in 2% negative goat serum overnight at 4 &(8)451;. Followed by secondary antibody Goat Anti-Rabbit IgG H&L (iFluor™ 488) at a dilution of 1:1,000. PBS instead of the primary an... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
PA5-144673
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant protein within human DRP1 aa 1-736/736.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 0.1% BSA, 40% glycerol
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive control: A549 cell lysate, 293 cell lysate, HCT116 cell lysate, Hela cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, NIH/3T3, 293T.
Predicted band size: 82 kDa
Subcellular Location: Cytoplasm, cytosol, Golgi apparatus, Endomembrane system, Mitochondrion outer membrane, Peroxisome, Membrane, clathrin-coated pit, Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane.
Target Information
A human dynamin-related protein, DRP1 contributes to mitochondrial division in mammalian cells. It plays this important role in mitochondrial fission at steady state and during apoptosis. DRP1 is required for proper cellular distribution of mitochondria, and in mutant neurons, mitochondria are largely absent from synapses, thus providing a genetic tool to assess the role of mitochondria at synapses.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: C-terminal region; DLP1; Dnm1p/Vps1p-like protein; dymple; dynamin family member proline-rich carboxyl-terminal domain less; dynamin-like protein 1; dynamin-like protein 4; dynamin-like protein IV; dynamin-related protein 1; DYNIV 11; DYNIV-11; EC 3.6.5.5; FLJ41912; large G-protein; N-terminal region; putative GTP-binding protein; unnamed protein product; VPS1
Gene Aliases: 6330417M19Rik; DLP1; Dnml1; Dnmlp1; DRP1; DVLP; DYMPLE; EMPF; EMPF1; HDYNIV; OPA5; python
UniProt ID: (Human) O00429, (Mouse) Q8K1M6, (Rat) O35303
Entrez Gene ID: (Human) 10059, (Mouse) 74006, (Rat) 114114
GTPase activity
GTPase activator activity
protein binding
GTP binding
microtubule binding
GTP-dependent protein binding
small GTPase binding
ubiquitin protein ligase binding
identical protein binding
protein homodimerization activity
clathrin binding
macromolecular complex binding
BH2 domain binding
protein serine/threonine kinase binding
mitochondrial fission
calcium ion transport
mitochondrion organization
regulation of gene expression
negative regulation of mitochondrial fusion
regulation of mitochondrion organization
peroxisome fission
metal ion transport
response to endoplasmic reticulum stress
synaptic vesicle recycling via endosome
mitochondrial fragmentation involved in apoptotic process
mitochondrion distribution
intracellular distribution of mitochondria
synaptic vesicle endocytosis
positive regulation of protein secretion
protein oligomerization
heart contraction
positive regulation of dendritic spine morphogenesis
protein localization to mitochondrion
cellular response to lipid
cellular response to hypoxia
positive regulation of neutrophil chemotaxis
positive regulation of mitochondrial fission
mitochondrial membrane fission
mitocytosis
regulation of peroxisome organization
positive regulation of synaptic vesicle endocytosis
regulation of mitophagy
regulation of ATP metabolic process
regulation of ubiquitin protein ligase activity
response to flavonoid
response to hypobaric hypoxia
positive regulation of synaptic vesicle exocytosis
endoplasmic reticulum organization
positive regulation of secretion by cell
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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