Invitrogen

DNP Monoclonal Antibody (LO-DNP-2)

Product Details

04-8300

Applications	Tested Dilution	Publications
Western Blot (WB)	-	View 1 publication 1 publication
Immunohistochemistry (IHC)	Assay-dependent	View 2 publications 2 publications
Immunohistochemistry (Paraffin) (IHC (P))	-	View 2 publications 2 publications
Immunocytochemistry (ICC/IF)	-	View 1 publication 1 publication
ELISA (ELISA)	Assay-dependent	-
Neutralization (Neu)	-	View 2 publications 2 publications
Miscellaneous PubMed (Misc)	-	View 1 publication 1 publication

Product Specifications
Species Reactivity	Chemical
Published species	Chemical
Host/Isotype	Rat / IgG1, kappa
Class	Monoclonal
Type	Antibody
Clone	LO-DNP-2
Immunogen	Synthetic DNP
Conjugate	Unconjugated
Form	Liquid
Concentration	1 mg/mL
Purification	purified
Storage buffer	PBS
Contains	0.1% sodium azide
Storage conditions	-20°C
RRID	AB_2532964

Target Information

DNP (2,4-dinitrophenol) is a toxic dye, chemically related to trinitrophenol (picric acid). It is used in biochemical studies of oxidative processes where it uncouples oxidative phosphorylation and is also used as a metabolic stimulant. 2,4-Dinitrophenol is used in the manufacture of dyes, wood preservatives, and as a pesticide. The acute (short-term) effects of 2,4-dinitrophenol in humans through oral exposure are nausea, vomiting, sweating, dizziness, headaches, and loss of weight. Chronic (long-term) oral exposure to 2,4-dinitrophenol in humans has resulted in the formation of cataracts and skin lesions, weight loss, and has caused effects on the bone marrow, central nervous system (CNS), and cardiovascular system. [National Center for Biotechnology Information. PubChem Compound Database; CID=1493].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A universal dual mechanism immunotherapy for the treatment of influenza virus infections.

Nature communications

Liu X,Zhang B,Wang Y,Haymour HS,Zhang F,Xu LC,Srinivasarao M,Low PS

04-8300 was used in Antibody recruitment to target the surface of free virus and viral-infected cells in an attempt to treat infections refractory to current therapies.

Thu Nov 05 00:00:00 EST 2020

KSHV attachment and entry are dependent on αVβ3 integrin localized to specific cell surface microdomains and do not correlate with the presence of heparan sulfate.

Virology

Garrigues HJ,DeMaster LK,Rubinchikova YE,Rose TM

04-8300 was used in Immunocytochemistry-immunoflourescence to demonstrate a critical role for αVΒ3 in the binding and entry of KSHV that is not shared with other proposed receptors.

Mon Sep 01 00:00:00 EDT 2014

A mutant light-chain ferritin that causes neurodegeneration has enhanced propensity toward oxidative damage.

Free radical biology & medicine

Baraibar MA,Barbeito AG,Muhoberac BB,Vidal R

04-8300 was used in Immunohistochemistry on paraffin embedded tissues to demonstrate an enhanced propensity of mutant ferritin to undergo iron-catalysed oxidative damage.

Tue May 01 00:00:00 EDT 2012

Monitoring autophagy in Alzheimer's disease and related neurodegenerative diseases.

Methods in enzymology

Yang DS,Lee JH,Nixon RA

04-8300 was used in immunohistochemistry to identify methods to monitor autophagy in neurodegenerative disorders

Thu Apr 02 00:00:00 EDT 2009

TA-270 [4-hydroxy-1-methyl-3-octyloxy-7-sinapinoylamino-2(1H)-quinolinone], an anti-asthmatic agent, inhibits leukotriene production induced by IgE receptor stimulation in RBL-2H3 cells.

The Journal of pharmacology and experimental therapeutics

Ishiwara M,Aoki Y,Takagaki H,Ui M,Okajima F

Sat Nov 01 00:00:00 EST 2003

TA-270 [4-hydroxy-1-methyl-3-octyloxy-7-sinapinoylamino-2(1H)-quinolinone], an anti-asthmatic agent, inhibits leukotriene production induced by IgE receptor stimulation in RBL-2H3 cells.

The Journal of pharmacology and experimental therapeutics

Ishiwara M,Aoki Y,Takagaki H,Ui M,Okajima F

Sat Nov 01 00:00:00 EST 2003

Paired activating and inhibitory immunoglobulin-like receptors, MAIR-I and MAIR-II, regulate mast cell and macrophage activation.

The Journal of experimental medicine

Yotsumoto K,Okoshi Y,Shibuya K,Yamazaki S,Tahara-Hanaoka S,Honda S,Osawa M,Kuroiwa A,Matsuda Y,Tenen DG,Iwama A,Nakauchi H,Shibuya A

04-8300 was used in blocking or activating experiment to identify and characterize myeloid-associated immunoglobulin-like receptor I and II

Mon Jul 21 00:00:00 EDT 2003

Caloric restriction of rhesus monkeys lowers oxidative damage in skeletal muscle.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Zainal TA,Oberley TD,Allison DB,Szweda LI,Weindruch R

04-8300 was used in immunohistochemistry to measure and localize the age-dependent accrual of oxidative damage in rhesus monkey vastus lateralis skeletal muscle

Fri Sep 01 00:00:00 EDT 2000

Cytochemical demonstration of oxidative damage in Alzheimer disease by immunochemical enhancement of the carbonyl reaction with 2,4-dinitrophenylhydrazine.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society

Smith MA,Sayre LM,Anderson VE,Harris PL,Beal MF,Kowall N,Perry G

04-8300 was used in immunohistochemistry - paraffin section to develop a cytochemical technique to specifically localize biomacromolecule-bound carbonyl reactivity