Proteintech

DR4 Polyclonal Antibody

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DR4 Antibody in Western Blot (WB) — Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 24063-1-AP ( DR4 Antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

Product Details

24063-1-AP

Applications	Tested Dilution	Publications
Western Blot (WB)	1:500-1:2,000	View 3 publications 3 publications
Flow Cytometry (Flow)	Assay-dependent	View 1 publication 1 publication

Product Specifications
Species Reactivity	Human
Published species	Human
Host/Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DR4 Fusion Protein Ag20927 (261-468 aa encoded by BC012866) View immunogen
Conjugate	Unconjugated
Form	Liquid
Concentration	0.2 mg/mL
Purification	Antigen affinity chromatography
Storage buffer	PBS, pH 7.3, with 50% glycerol
Contains	0.02% sodium azide
Storage conditions	-20°C

Product Specific Information

Immunogen sequence: CCCIGSGCG GDPKCMDRVC FWRLGLLRGP GAEDNAHNEI LSNADSLSTF VSEQQMESQE PADLTGVTVQ SPGEAQCLLG PAEAEGSQRR RLLVPANGAD PTETLMLFFD KFANIVPFDS WDQLMRQLDL TKNEIDVVRA GTAGPGDALY AMLMKWVNKT GRNASIHTLL DALERMEERH AKEKIQDLLV DSGKFIYLED GTGSAVSLE (261-468 aa encoded by BC012866)

Target Information

TRAIL-R1 (CD261, DR4) is a type I transmembrane protein, also called TRAIL receptor 1. The ligand for this DR4 death receptor has been identified and termed TRAIL, which is a member of the TNF family. DR4, as many other receptors (Fas, TNFR1, etc.), mediates apoptosis and NF kappaB activation in many cells and tissues. Apoptosis, a programmed cell death, is a operating process in normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by coupled of certain cytokines (TNF family - TNF, Fas ligand) and their death domain containing receptors (TNFR1, Fas receptor).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Jinfukang induces cellular apoptosis through activation of Fas and DR4 in A549 cells.

Oncology letters

Lu J,Chen J,Kang Y,Wu J,Shi H,Fu Y,Jiao L,Dong C,Li X,Jin Y,Zhao W,Xu L,Zhao X

Mon Oct 01 00:00:00 EDT 2018

Jinfukang induces cellular apoptosis through activation of Fas and DR4 in A549 cells.

Oncology letters

Lu J,Chen J,Kang Y,Wu J,Shi H,Fu Y,Jiao L,Dong C,Li X,Jin Y,Zhao W,Xu L,Zhao X

Mon Oct 01 00:00:00 EDT 2018

JWA regulates TRAIL-induced apoptosis via MARCH8-mediated DR4 ubiquitination in cisplatin-resistant gastric cancer cells.

Oncogenesis

Wang Q,Chen Q,Zhu L,Chen M,Xu W,Panday S,Wang Z,Li A,Røe OD,Chen R,Wang S,Zhang R,Zhou J

Mon Jul 03 00:00:00 EDT 2017

Celastrol induces apoptosis and autophagy via the ROS/JNK signaling pathway in human osteosarcoma cells: an in vitro and in vivo study.

Cell death & disease

Li HY,Zhang J,Sun LL,Li BH,Gao HL,Xie T,Zhang N,Ye ZM

Thu Jan 22 00:00:00 EST 2015

Bioinformatics

Protein Aliases: CD261; cytotoxic TRAIL receptor; Death receptor 4; MGC9365; sCD261; soluble CD261; soluble TRAIL; sTRAIL; TNF-related apoptosis-inducing ligand receptor 1; TRAIL R1; TRAIL receptor 1; TRAIL-R1; Tumor necrosis factor receptor superfamily member 10A; tumor necrosis factor receptor superfamily member 10a variant 2; tumor necrosis factor receptor superfamily, member 10a

Gene Aliases: APO2; CD261; DR4; TNFRSF10A; TRAILR-1; TRAILR1

UniProt ID: (Human) O00220

Entrez Gene ID: (Human) 8797

Molecular Function: transmembrane signal receptor