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Invitrogen
DRAM Polyclonal Antibody
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FIGURE: 1 / 5
DRAM Antibody (PA5-20335) in IHC (P)
Immunohistochemical analysis of paraffin-embedded human liver tissue using DRAM Polyclonal Antibody (Product # PA5-20335) at 2.5 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. ... View More
Product Details
PA5-20335
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A 16 amino acid peptide from near the carboxy terminus of human DRAM.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
A suggested positive control is 293 cell lysate.
PA5-20335 can be used with blocking peptide PEP-0455.
Target Information
Damage-regulated autophagy modulator (DRAM) is a p53 target gene encoding a lysosomal protein that induces autophagy, a process that degrades cytosolic proteins and organelles. It has been suggested that activation of DRAM by p53 is simultaneous to the activation by p53 of one or more proapoptotic genes such as PUMA, Bax, etc., and that the signaling pathways regulated by these genes together promote a full cell death response. By itself, DRAM cannot induce apoptosis, but the fact that it is inactivated in certain cancers highlights the importance of DRAM and suggests that autophagy may play a more important role in cancer than initially suspected. At least two different isoforms of DRAM are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Damage-regulated autophagy modulator; DNA damage-regulated autophagy modulator protein 1; DNA-damage regulated autophagy modulator 1; FLJ11259
Gene Aliases: 1200002N14Rik; DRAM; DRAM1
UniProt ID: (Human) Q8N682, (Mouse) Q9DC58
Entrez Gene ID: (Human) 55332, (Mouse) 71712, (Rat) 679937
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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