Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is brain tissue.
The D1 subtype is the most abundant dopamine receptor in the central nervous system. This G-protein coupled receptor stimulates adenylyl cyclase and activates cyclic AMP-dependent protein kinases. D1 receptors regulate neuronal growth and development, mediate some behavioral responses, and modulate dopamine receptor D2-mediated events. Alternate transcription initiation sites result in two transcript variants of this gene.
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Protein Aliases: D(1B) dopamine receptor; D(5) dopamine receptor; D1b receptor; D1beta dopamine receptor; Dopamine 5 receptor; Dopamine D5 receptor; dopamine receptor D1B; DRD 1B; DRD 5; Hd5dr; MGC10601
Gene Aliases: DBDR; DRD1B; DRD1L2; DRD5
UniProt ID: (Human) P21918
Entrez Gene ID: (Human) 1816
Molecular Function: G-protein coupled receptor