Immunofluorescent analysis of Desmin (green) in cultured primary cardiomyocytes. Primary cardiomyocytes were isolated and cultured using the Primary Cardiomyocyte Isolation Kit (Product # 88281). Cells were grown in 24-well plates (seeded at 5 x 105 cells/well) or in 35mm glass bottom plates (at a density of 2.5 x 106 cells/well). At day 1 and day 7, cells were fixed with 4% paraformaldehyde, permeablilized with 0.1% Triton X-100 in HBSS for 10 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were probed with a Desmin polyclonal antibody (Product # PA1-37556) at dilution of 1:300 for 2 hours at room temperature or overnight at 4 °C, washed with HBSS, and incubated with a DyLight 488-conjugated goat anti-rabbit IgG secondary antibody (Product # 35552) at dilution of 1:500 for 1 hour at room temperature. Nuclei (blue) were visualized using Hoechst 33342 (Product # 62249). Images were taken at 20X magnification on a Carl Zeiss microscope (AxioVision Rel. 4.7).
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Mouse, Not Applicable|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide mapping near the C-terminus of human desmin|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.6, with 1% BSA|
|Contains||<0.1% sodium azide|
|Storage Conditions||4° C, do not freeze|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:200|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
This antibody is predicted to react with bovine, chicken, canine, amphibian, porcine, rabbit and rat based on sequence homology.
A suggested positive control is skeletal muscle or sarcoma.
Desmin is a 53 kDa intermediate filament protein expressed in cardiac, skeletal, and smooth muscle cells, as well as lung tissue. In skeletal and cardiac muscle cells, desmin is localized at the Z-disk region and the intercalated disk. In smooth muscle tissue, desmin is expressed throughout the cells. During development, levels of desmin expression vary, being present at low levels in the early stages of myoblast replication and increasing during terminal differentiation. Unlike vimentin, desmin is expressed in both differentiating and mature muscle cells. Inhibiting desmin expression blocks myoblast fusion and myotube formation, suggesting that desmin is crucial during myogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Resolving Discrepant Findings on ANGPTL8 in ß-Cell Proliferation: A Collaborative Approach to Resolving the Betatrophin Controversy.
PA1-37556 was used in immunohistochemistry - paraffin section to use a blinded study to examine the effects of ANGPTL8 upon beta-cell proliferation
|Cox AR,Barrandon O,Cai EP,Rios JS,Chavez J,Bonnyman CW,Lam CJ,Yi P,Melton DA,Kushner JA||PloS one (11:null)||2016|
|Not Applicable||Not Cited||
Rosmarinic acid and baicalin epigenetically derepress peroxisomal proliferator-activated receptor ¿ in hepatic stellate cells for their antifibrotic effect.
PA1-37556 was used in immunohistochemistry to investigate the mechanism for the protective effect of herbal prescription Yang-Gan-Wan against liver fibrosis
|Yang MD,Chiang YM,Higashiyama R,Asahina K,Mann DA,Mann J,Wang CC,Tsukamoto H||Hepatology (Baltimore, Md.) (55:1271)||2012|