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Invitrogen
Desmoglein 3 Polyclonal Antibody
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FIGURE: 1 / 2
Desmoglein 3 Antibody (PA5-79172) in WB
Western blot was performed using Anti-Desmoglein 3 Polyclonal Antibody (Product # PA5-79172) and a 130 kDa band corresponding to Desmoglein-3 was observed across cell lines tested except MCF-7, PC-3 and DU145 which is reported to be low. Membrane enriched extracts (30 µg lysate) of A-431 (Lane 1), MCF 10A (Lane 2), MCF7 (Lane 3), PC-3 (Lane 4) and DU 145 (Lane 5) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by ... View More
Product Details
PA5-79172
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human Desmoglein 3 (981-999aa QLRGSHTMLCTEDPCSRLI ).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
no preservative
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human A431 whole cell, human Hacat whole cell.
Target Information
Desmoglein 3 (Dsg3), also known as Cadherin family member 6 (CDHF6), is a member of the desmosomal cadherin family and plays a critical role in cell-cell adhesion. It is predominately expressed in stratified squamous epithelium, including the skin, oral mucosa, and esophagus. Desmoglein 3 has been characterized as the auto-antigen of pemphigus vulgaris (PV), a life-threatening autoimmune disease. Binding of anti-Desmoglein 3 autoantibodies causes the loss of cell-cell adhesion, which clinically manifests as blisters on the skin and oral mucosa, and histologically as suprabasilar acantholysis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 130 kDa pemphigus vulgaris antigen; Cadherin family member 6; Desmoglein-3; DKFZp686P23184; PVA; unnamed protein product
Gene Aliases: ABOLM; CDHF6; DSG3; PVA
UniProt ID: (Human) P32926
Entrez Gene ID: (Human) 1830
desmosome assembly
actin filament organization
cell adhesion
homophilic cell adhesion via plasma membrane adhesion molecules
negative regulation of cell migration
regulation of protein stability
adherens junction assembly
cell-cell adhesion
tight junction assembly
positive regulation of bicellular tight junction assembly
negative regulation of p38MAPK cascade
positive regulation of protein localization to adherens junction
positive regulation of protein localization to membrane
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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