Immunofluorescent analysis of Dishevelled 3 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min and stained using a Dishevelled 3 polyclonal antibody (Product # PA5-27723) diluted at 1:500. Blue: Hoechst 33342 staining.
|Tested species reactivity||Mouse, Human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Recombinant fragment corresponding to a region within amino acids 1 and 166 of Human Dishevelled 3|
|Purification||Antigen affinity chromatography|
|Storage buffer||0.1M tris glycine, pH 7, with 10% glycerol|
|Storage Conditions||-20° C, Avoid Freeze/Thaw Cycles|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:100-1:1000|
|Western Blot (WB)||1:5000-1:20,000|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
PA5-27723 targets Dishevelled 3 in IF, IHC, IHC (P), and WB applications and shows reactivity with Human and Mouse samples.
The PA5-27723 immunogen is recombinant fragment corresponding to a region within amino acids 1 and 166 of Human Dishevelled 3.
This gene is a member of a multi-gene family which shares strong similarity with the Drosophila dishevelled gene, dsh. The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation.
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