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Agrisera
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Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Yersinia pseudotuberculosis YPIII/pIB102 (serotype III)
In prokaryotes, the heat shock response is characterized by the induction of several proteins including DnaJ, DnaK, GroEL and GroES. DnaK is a member of the HSP70 family, and like other members, exhibits ATPase activity which facilitates the release of bound proteins and disaggregation of protein complexes within the cell. Members of this protein group serve as molecular chaperones. Levels of DnaJ and DnaK are closely linked and, in E. coli, are constituents of a single operon. DnaJ regulates DnaK in several different systems. Relative to DnaK, the level of DnaJ synthesis is quite low. Studies have shown that DnaJ and DnaK, together with GrpE, cooperatively interact with firefly luciferase as it unfolds during thermal denaturation allowing renaturation of the protein upon return to normal temperatures. Also, DnaK together with DnaJ stimulates the binding of RepA initiator protein to the origin of P1 phage in vitro.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CPR3; DNAJA2; DNJ3; Escherichia coli; HDJ-2
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