Immunohistochemistry analysis of E-cadherin / CDH1 (NCH-38) showing staining in the membrane and weak cytoplasm of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a E-cadherin / CDH1 Antibody (NCH-38) Mouse Monoclonal Antibody (MA512547) diluted in 3% BSA-PBS at a dilution of 1:20 for 1 hour at 37°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
|Tested species reactivity||Human, Mouse|
|Published species reactivity||Rat, Human|
|Host / Isotype||Mouse / IgG1, kappa|
|Immunogen||E-cadherin and GST recombinant protein|
|Storage buffer||tissue culture supernatant|
|Contains||0.09% sodium azide|
|Storage Conditions||4° C|
|Tested Applications||Dilution *|
|Immunohistochemistry (Paraffin) (IHC (P))||1:20|
|Western Blot (WB)||2-4 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
MA5-12547 targets E-Cadherin in IHC (P) applications and shows reactivity with Human samples.
The MA5-12547 immunogen is e-cadherin and GST recombinant protein.
E-cadherin (uvomorulin, cell-CAM120/80) is a calcium dependent cell adhesion molecule expressed predominately in epithelial tissues. It plays an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Numerous studies have demonstrated that reduction and/or loss of E-cadherin expression in carcinomas correlates positively with the potential of these tumors for invasion and metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Malignant transformation of adenomyoepithelioma of the breast by a monophasic population: a report of two cases and review of literature.
MA5-12547 was used in immunohistochemistry to report on two cases of malignant transformation of adenomyoepithelioma of the breast by a monophasic population
|Marian C,Boila A,Soanca D,Malau M,Podeanu DM,Resetkova E,Stolnicu S||APMIS : acta pathologica, microbiologica, et immunologica Scandinavica (121:272)||2013|
Five primary human pancreatic adenocarcinoma cell lines established by the outgrowth method.
MA5-12547 was used in immunohistochemistry to establish and characterize five new pancreatic adenocarcinoma cell lines
|Rückert F,Aust D,Böhme I,Werner K,Brandt A,Diamandis EP,Krautz C,Hering S,Saeger HD,Grützmann R,Pilarsky C||The Journal of surgical research (172:29)||2012|
Nogo-A inhibitory peptide (NEP1-40) increases pan-cadherin expression following mild cortical contusion injury in rats.
MA5-12547 was used in immunohistochemistry to study the effects of a Nogo-A neutralizing peptide on cadherin expression in a rat model of mild cerebral contusion
|Atalay B,Bavbek M,Ozen O,Nacar A,Gül¿en S,Yi¿itkanli K,Caner H,Altinörs N||Turkish neurosurgery (18:356)||2008|
The expression of cytoskeleton regulatory protein Mena in colorectal lesions.
MA5-12547 was used in immunohistochemistry to investigate the expression of mammalian actin regulatory protein Ena in colorectal lesions
|Gurzu S,Jung I,Prantner I,Ember I,Pávai Z,Mezei T||Romanian journal of morphology and embryology = Revue roumaine de morphologie et embryologie (49:345)||2008|
The expression of cytokeratin 5/6 in invasive lobular carcinoma of the breast: evidence of a basal-like subset?
MA5-12547 was used in immunohistochemistry to investigate the expression of cytokeratin 5/6 in a subset of invasive lobular carcinoma of the breast and its prognostic significance
|Fadare O,Wang SA,Hileeto D||Human pathology (39:331)||2008|
Cystic hypersecretory carcinoma: rare and poorly recognized variant of intraductal carcinoma of the breast. Report of five cases.
MA5-12547 was used in immunohistochemistry to report the clinical cases of cystic hypersecretory carcinoma
|Skalova A,Ryska A,Kajo K,Di Palma S,Kinkor Z,Michal M||Histopathology (46:43)||2005|
Human ovarian surface epithelial cells immortalized with hTERT maintain functional pRb and p53 expression.
MA5-12547 was used in immunocytochemistry to develop an imortalized human ovarian surface epithelial cell line that maintains functional pRb and p53 expression
|Li NF,Broad S,Lu YJ,Yang JS,Watson R,Hagemann T,Wilbanks G,Jacobs I,Balkwill F,Dafou D,Gayther SA||Cell proliferation (40:780)||2007|