Western blot analysis of Mouse Brain Tissue Lysate using PA5-19709, EAAT1 primary antibody at a dilution of 1 ug/ml (lane 1). PA5-19709 staining of Mouse Brain Tissue Lysate at a dilution of 2 ug/ml (lane 2). Blot treated with a secondary IR Dye680-conjugated Goat polyclonal anti-Rabbit antibody was used at a dilution of 1:10000.
|Tested species reactivity||Human, Mouse, Rat|
|Published species reactivity||Mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human EAAT1.|
|Purification||Antigen affinity chromatography|
|Storage buffer||PBS, pH 7.4, with 1% BSA|
|Contains||0.02% sodium azide|
|Storage Conditions||Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.|
|Tested Applications||Dilution *|
|Immunocytochemistry (ICC)||5 µg/ml|
|Immunofluorescence (IF)||5 µg/ml|
|Immunohistochemistry (Paraffin) (IHC (P))||1:50|
|Western Blot (WB)||1 µg/ml|
* Suggested working dilutions are given as a guide only. It is recommended that the user titrate the product for use in their own experiment using appropriate negative and positive controls.
|Miscellaneous PubMed (MISC)||See 1 publications below|
Not suitable for use with IHC (PFA) or IHC (F).
This antibody is predicted to react with rat and cow based on sequence homology.
This gene encodes a member of a member of a high affinity glutamate transporter family. Mutations in this gene are associated with episodic ataxia, Type 6. Alternative splicing results in multiple transcript variants.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Effects of sleep and wake on astrocytes: clues from molecular and ultrastructural studies.
PA5-19709 was used in western blot to examine the effects of sleep and wake on astrocytes
|Bellesi M,de Vivo L,Tononi G,Cirelli C||BMC biology (13:null)||2015|