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Bethyl Laboratories
EB1 Polyclonal Antibody
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FIGURE: 1 / 1
EB1 Antibody (A302-331A) in WB
Detection of human EB1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-EB1 antibody (Product # A302-331A) used for WB at 0.04 µg/mL (A) and 1 µg/mL (B) and used for IP at 10 µg/mg lysate. EB1 was also immunoprecipitated by rabbit anti-EB1 antibodies A302-332A and A302-333A, which recognize downstream epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 se... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
A302-331A
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 125 and 175 of human End-binding protein 1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.20 mg/mL
Amount
20 µg
Purification
Antigen affinity chromatography
Storage buffer
TBS, pH 7.0 to 8.0, with 0.1% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
EB1 family proteins are evolutionarily conserved proteins that bind microtubule plus-ends and centrosomes and regulate the dynamics and organization of microtubules. The MAPRE genes encode the EB1 family proteins. The human MAPRE genes MAPRE1, MAPRE2, and MAPRE3 code for EB1, RP1, and EBF3 proteins, respectively. EB1 family members appear to be expressed ubiquitously. The EB1 (MAPRE1) gene, at 20q11. 2, is fused to MLL in an adult patient with pro-B acute lymphoblastic leukemia. EB1 is a microtubule-associated protein that interacts with the colorectal adenomatous polyposis coli tumor-suppressor protein and plays important roles in regulating microtubule dynamics, cell polarity, and chromosome stability.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: adenomatous polyposis coli-binding protein EB1; APC-binding protein EB1; end-binding protein 1; MGC117374; MGC129946; unnamed protein product
Gene Aliases: EB1
UniProt ID: (Human) Q15691
Entrez Gene ID: (Human) 22919
establishment of mitotic spindle orientation
microtubule bundle formation
protein localization
cell migration
regulation of microtubule polymerization or depolymerization
negative regulation of microtubule polymerization
positive regulation of microtubule polymerization
protein localization to microtubule
microtubule polymerization
spindle assembly
attachment of mitotic spindle microtubules to kinetochore
protein localization to mitotic spindle
protein localization to astral microtubule
positive regulation of cellular protein localization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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