Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 3
EEF1A2 Antibody (600-101-ML6) in ICC/IF
Immunofluorescence of Goat Anti-EEF1A2 Antibody. Cell Line: MCF-7 cells. Fixative: 100% MeOH. Permeabilization: 0.3% Triton X-100. Primary Antibody: Anti-EEF1A2 at 15µg/mL overnight at 2-8°C. Secondary Antibody: Donkey Anti-Goat DyLight™488 Antibody (p/n 605-741-125) at 5µg/mL for 1 hr at RT. Nuclear Counterstain: DAPI. Staining: (A) DAPI. (B) Anti-EEF1A2 + secondary. (C) merge A+B. (D). Secondary Only. Localization Expected: nucleoli, cytosol.
Product Details
600-101-ML6
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Anti-EEF1A2 antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near C-terminal portion of human EEF1A2 conjugated to Keyhole Limpet Hemocyanin (KLH).
Conjugate
Form
Liquid
Concentration
0.77 mg/mL
Purification
Affinity chromatography
Storage buffer
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
Contains
0.01% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.^M
^M
This affinity purified antibody is directed against human EEF1A2. A BLAST analysis was used to suggest cross-reactivity with the antigen based on 100% homology with the immunizing sequence to human, mouse, rat, bovine, and rabbit.
Target Information
This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. [provided by RefSeq, Mar 2014]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: eEF1A-2; EF-1-alpha-2; Elongation factor 1-alpha 2; Eukaryotic elongation factor 1 A-2; Statin-S1
Gene Aliases: Eef1a; EEF1A2; EEF1AL; EF-1-alpha-2; EF1A; EIEE33; HS1; MRD38; S1; STN; STNL; wasted; wst
UniProt ID: (Human) Q05639, (Mouse) P62631
Entrez Gene ID: (Human) 1917, (Mouse) 13628
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support